Yue Jiao, Tan Yuqi, Wei Rujian, Wang Xu, Mubeen Samavia, Chen Canni, Cao Shan, Wang Caijin, Chen Peng
College of Agriculture, Guangxi University, Nanning, 530004 China.
Key Laboratory of Crop Genetic Breeding and Germplasm Innovation, Nanning, 530004 China.
Physiol Mol Biol Plants. 2024 Sep;30(9):1517-1532. doi: 10.1007/s12298-024-01504-y. Epub 2024 Aug 28.
Among plants' transcription factor families, the bHLHs family has a significant influence on plant development processes and stress tolerance. However, there have been no relevant studies performed on the bHLHs family in kenaf ( L). Here, the bHLH transcription factors in kenaf were found using bioinformatics, and a total of 141 kenaf transcription factors were identified. Phylogenetic analysis revealed that these transcription factors were irregularly distributed on 18 chromosomes and separated into 20 subfamilies. Additionally, utilizing the transcriptome data under diverse abiotic pressures, the expression of members was analyzed under different stress conditions. A typical abiotic stress transcription factor, , was exposed to salt, drought, heavy metals, and ABA. The findings revealed that might be activated under salt, drought, cadmium stress, and ABA conditions. Furthermore, 's function under salt stress conditions was studied after it was silenced using the virus-induced gene silencing (VIGS) technique. Reduced antioxidant enzyme activity and stunted plant development were seen in VIGS-silenced seedlings. Stress-related genes were shown to be considerably downregulated in the -silenced kenaf plants, according to the qRT-PCR study. In conclusion, this study provides the first systematic gene family analysis of the kenaf bHLH gene family and provides a preliminary validation of the salt tolerance function of the gene. This study lays the foundation for future research on the regulatory mechanisms of bHLH genes in response to abiotic stresses.
The online version contains supplementary material available at 10.1007/s12298-024-01504-y.
在植物转录因子家族中，bHLHs家族对植物发育过程和胁迫耐受性有重大影响。然而，目前尚未对红麻（L.）中的bHLHs家族进行相关研究。在此，利用生物信息学方法在红麻中发现了bHLH转录因子，共鉴定出141个红麻转录因子。系统发育分析表明，这些转录因子不规则地分布在18条染色体上，并分为20个亚家族。此外，利用不同非生物胁迫下的转录组数据，分析了各成员在不同胁迫条件下的表达情况。一个典型的非生物胁迫转录因子，在盐、干旱、重金属和脱落酸处理下进行了研究。结果表明，在盐、干旱、镉胁迫和脱落酸条件下可能被激活。此外，利用病毒诱导基因沉默（VIGS）技术沉默后，研究了其在盐胁迫条件下的功能。在VIGS沉默的幼苗中观察到抗氧化酶活性降低和植株发育受阻。根据qRT-PCR研究，在沉默的红麻植株中，与胁迫相关的基因显著下调。总之，本研究首次对红麻bHLH基因家族进行了系统的基因家族分析，并对基因的耐盐性功能进行了初步验证。本研究为今后研究bHLH基因响应非生物胁迫的调控机制奠定了基础。
在线版本包含可在10.1007/s12298-024-01504-y获取的补充材料。
