China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, PR China.
Int J Syst Evol Microbiol. 2024 Sep;74(9). doi: 10.1099/ijsem.0.006525.
One bacterial strain, designated as C22-A2, was isolated from Lake LungmuCo in Tibet. Cells of strain C22-A2 were long rod-shaped, Gram-stain-negative, non-spore-forming, with positive catalase and oxidase activity. Optimal growth occurred at 20-25 °C, pH 8.0 and with 3.0-7.0% (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene and whole genome sequences revealed that strain C22-A2 belonged to the genus , showing the highest 16S rRNA gene similarity to DSM 10037 (97.6%). The average nucleotide identity values between strain C22-A2 and the type strains of related species in the genus were less than 74.4% and the digital DNA-DNA hybridization values were less than 20.2%, both below the species delineation thresholds of 95 and 70% respectively. The genome analysis revealed that strain C22-A2 harboured genes responsible for osmotic and oxidative stress, enabling it to adapt to its surrounding environment. In terms of biochemical and physiological characteristics, strain C22-A2 shared similar characteristics with the genus including the predominant cellular fatty acid anteiso-C, the major respiratory quinone MK-7, as well as the polar lipids phosphatidylglycerol and diphosphatidylglycerol. Based on the comprehensive analysis of phylogenetic, phylogenomic, morphological, physiological and biochemical characteristics, strain C22-A2 is proposed to represent a novel species of the genus , named as sp. nov. (=CGMCC 1.19202=KCTC 43426).
一株细菌菌株,被命名为 C22-A2,从西藏的 LungmuCo 湖分离得到。C22-A2 菌株的细胞呈长杆状,革兰氏阴性,不产芽孢,具有阳性过氧化氢酶和氧化酶活性。最佳生长条件为 20-25°C、pH8.0 和 3.0-7.0%(w/v)NaCl。16S rRNA 基因和全基因组序列的系统发育分析表明,菌株 C22-A2 属于 属,与 DSM 10037(97.6%)的 16S rRNA 基因相似度最高。菌株 C22-A2 与该属中相关种的模式菌株之间的平均核苷酸同一性值均小于 74.4%,数字 DNA-DNA 杂交值均小于 20.2%,均低于 95%和 70%的种系划分阈值。基因组分析表明,菌株 C22-A2 具有应对渗透和氧化应激的基因,使其能够适应周围环境。在生化和生理特性方面,菌株 C22-A2 与 属具有相似特征,包括主要的细胞脂肪酸 anteiso-C、主要的呼吸醌 MK-7 以及极性脂磷脂酰甘油和双磷脂酰甘油。基于系统发育、系统基因组学、形态学、生理学和生化特性的综合分析,菌株 C22-A2 被提议代表 属的一个新种,命名为 sp. nov.(=CGMCC 1.19202=KCTC 43426)。