Gabrielian S A, Bogdanova L F, Vorob'eva L S, Givental' N I
Antibiot Med Biotekhnol. 1985 Jul;30(7):511-6.
Sensitivity of 200 strains of Pseudomonas aeruginosa to 8 antibiotics was studied with 2 methods, agar dilution and agar diffusion. The data obtained with the two methods were in good agreement. The simple method of agar diffusion provided sufficiently precise results in determination of the Pseudomonas sensitivity to carbenicillin and polymyxin. With the use of the correction principle by the "mobile intermediate zone" it also provided sufficiently precise results in determination of the Pseudomonas sensitivity to gentamicin. The necessity of using the reference strain ATCC 27853 of P. aeruginosa in every experiment is stressed. The peculiarities of the data interpretation in determination of antibiotic sensitivity of Pseudomonas are discussed.
采用琼脂稀释法和琼脂扩散法两种方法研究了200株铜绿假单胞菌对8种抗生素的敏感性。两种方法所获数据吻合良好。琼脂扩散这种简单方法在测定铜绿假单胞菌对羧苄青霉素和多粘菌素的敏感性时能提供足够精确的结果。运用“移动中间带”校正原理，该方法在测定铜绿假单胞菌对庆大霉素的敏感性时也能提供足够精确的结果。强调了在每个实验中使用铜绿假单胞菌参考菌株ATCC 27853的必要性。讨论了在测定铜绿假单胞菌抗生素敏感性时数据解读的特点。
