Woolfrey B F, Fox J M, Quall C O
Am J Clin Pathol. 1981 Jan;75(1):39-44. doi: 10.1093/ajcp/75.1.39.
This investigation compares minimum inhibitory concentration measurements by three antimicrobic dilution methods for Pseudomonas aeruginosa versus seven antimicrobics. Minimum inhibitory concentrations were measured for 650 P. aeruginosa clinical isolates and for repeated tests with P. aeruginosa ATCC 27853 versus gentamicin, tobramycin, amikacin, netilmicin, sisomicin, carbenicillin, and ticarcillin, using the macro-broth, micro-broth, and agar dilution methods. For all antimicrobics, it was found that the micro-broth and agar dilution methods produced comparable minimum inhibitory concentration measurements, which were found to lie 1 to 2 double dilution steps below those determined by the macro-broth method. Acceptably replicability was found for both the macro-broth and the agar dilution methods. The micro-broth method showed less replicability, with 4.7% of minimum inhibitory concentration values lying +/- 2 or more double dilution steps from the modal value. It is important to recognize such differences if micro-broth or agar dilution methods are substituted for the macro-broth method.
本研究比较了三种抗微生物稀释法测定铜绿假单胞菌对七种抗微生物药物的最低抑菌浓度。采用常量肉汤稀释法、微量肉汤稀释法和琼脂稀释法，对650株铜绿假单胞菌临床分离株以及铜绿假单胞菌ATCC 27853与庆大霉素、妥布霉素、阿米卡星、奈替米星、西索米星、羧苄西林和替卡西林进行重复试验，测定最低抑菌浓度。对于所有抗微生物药物，发现微量肉汤稀释法和琼脂稀释法得出的最低抑菌浓度测定结果相当，且比常量肉汤稀释法测定的结果低1至2个稀释倍数。常量肉汤稀释法和琼脂稀释法均具有可接受的重复性。微量肉汤稀释法的重复性较差，4.7%的最低抑菌浓度值与模态值相差±2个或更多稀释倍数。如果用微量肉汤稀释法或琼脂稀释法替代常量肉汤稀释法，认识到这些差异很重要。
