Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan.
Graduate Institute of Biomedical Science, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan.
Nanomedicine (Lond). 2024;19(28):2375-2390. doi: 10.1080/17435889.2024.2403326. Epub 2024 Sep 25.
Mineralo-organic particles, naturally present in human body fluids, participate in ectopic calcification and inflammatory diseases. These particles coexist with influenza A virus (IAV) in the same microenvironment during viral infection. Our objective was to investigate the functional consequences of the potential interactions between these particles and the virions. We used models, including electron microscopy, fluorescence microscopy, hemagglutination assay and viral infection assays to examine the interactions. Mineralo-organic particles bind to IAV virions through interactions involving particle-bound fetuin-A and mineral content, effectively engaging viral hemagglutinin. These interactions result in hindered viral infection. These findings uncover the novel interactions between mineralo-organic particles and IAV, highlighting the impact of virus microenvironment complexity.
矿化有机物颗粒天然存在于人体体液中，参与异位钙化和炎症性疾病。在病毒感染过程中，这些颗粒与甲型流感病毒（IAV）共存于相同的微环境中。我们的目的是研究这些颗粒与病毒之间潜在相互作用的功能后果。我们使用包括电子显微镜、荧光显微镜、血凝试验和病毒感染试验在内的模型来研究相互作用。矿化有机物颗粒通过颗粒结合的胎球蛋白-A 和矿物质含量与 IAV 病毒颗粒相互作用，有效地结合病毒血凝素。这些相互作用导致病毒感染受阻。这些发现揭示了矿化有机物颗粒与 IAV 之间的新相互作用，强调了病毒微环境复杂性的影响。
