Muniz Paulo Henrique Pereira Costa, de Oliveira Thiago Alves Santos, Duarte Elizabeth Amélia Alves, Rodrigues Fabrício, Carvalho Daniel Diego Costa
Universidade Estadual de Goiás, Ipameri, Goiás, Brasil.
Universidade Federal do Acre, Cruzeiro do Sul, Acre, Brasil.
Braz J Microbiol. 2024 Dec;55(4):3521-3528. doi: 10.1007/s42770-024-01520-w. Epub 2024 Sep 25.
Studies on physiological responses to stimuli from physical factors are essential for understanding the dynamics of the microorganisms and higly important for the management of plant diseases. Besides, the development of an epidemiological model for pathogen populations requires studying their physiological responses to physical stimuli. The objective of this study was to evaluate the germination dynamics of spores from six isolates of Bipolaris bicolor under effects of light at 25 °C. Suspensions of 1.6 × 10 conidia mL from the B. bicolor isolates were inoculated onto Petri dishes containing agar-water culture medium and incubated in a BOD chamber under two physical conditions: (a) constant darkness and (b) constant light for five hours. The study was conducted in a completely randomized design, with a 6 × 2 factorial arrangement (six B. bicolor isolates and two physical conditions) and five replications. The length of the germ tube was measured hourly. The constant darkness resulted in higher mean germ tube growth for the pathogen; however, differences in the final germination percentage were found among the isolates. The isolate F-24-02 exhibited the highest germination adaptability to constant darkness, presenting the longest germ tube length.
研究微生物对物理因素刺激的生理反应对于理解微生物的动态变化至关重要，对植物病害的管理也极为重要。此外，建立病原菌群体的流行病学模型需要研究它们对物理刺激的生理反应。本研究的目的是评估在25°C光照条件下，六种双极旋孢腔菌分离株孢子的萌发动态。将来自双极旋孢腔菌分离株的1.6×10分生孢子/mL悬浮液接种到含有琼脂-水培养基的培养皿中，并在两种物理条件下于生化培养箱中培养5小时：(a) 持续黑暗和(b) 持续光照。本研究采用完全随机设计，6×２析因排列（六种双极旋孢腔菌分离株和两种物理条件），重复五次。每小时测量芽管长度。持续黑暗导致病原菌的平均芽管生长更高；然而，各分离株的最终萌发率存在差异。分离株F-24-02对持续黑暗表现出最高的萌发适应性，芽管长度最长。
