Shaw K J, Campeau J D, Roche P C, diZerega G S
Exp Clin Endocrinol. 1985 Aug;86(1):26-34. doi: 10.1055/s-0029-1210469.
Fibrinolytic activity in porcine follicular fluid and plasminogen activator production by porcine granulosa cells in response to gonadotropins is described. Fibrinolytic activity was determined using a solid phase assay in which 125I-fibrinogen was coupled to latex beads. Urokinase plus plasminogen served as the standard. Porcine follicular fluid contained protease inhibitors as evidenced by its ability to inhibit the activity of an urokinase-plasminogen standard. Removal of these inhibitors by acid precipitation revealed plasminogen-dependent and -independent fibrinolytic activity. Cultured granulosa cells produced plasminogen activator in amounts that increased over time. This pattern was most significant in cells stimulated by hCG. Cells incubated with hCG produced significantly more plasminogen activator when compared to those cells cultured in absence of gonadotropin. The enhancement was most marked during the second 48 hrs in culture (p = 0.032). FSH was found to have no stimulatory effect on plasminogen activator production by cultured porcine granulosa cells.
本文描述了猪卵泡液中的纤溶活性以及猪颗粒细胞响应促性腺激素产生纤溶酶原激活物的情况。纤溶活性通过一种固相测定法来确定，其中125I - 纤维蛋白原与乳胶珠偶联。尿激酶加纤溶酶原作为标准品。猪卵泡液含有蛋白酶抑制剂，这可通过其抑制尿激酶 - 纤溶酶原标准品活性的能力得到证明。通过酸沉淀去除这些抑制剂后，显示出纤溶酶原依赖性和非依赖性纤溶活性。培养的颗粒细胞产生的纤溶酶原激活物量随时间增加。这种模式在由人绒毛膜促性腺激素（hCG）刺激的细胞中最为显著。与在无促性腺激素条件下培养的细胞相比，用hCG孵育的细胞产生的纤溶酶原激活物明显更多。这种增强在培养的第二个48小时内最为明显（p = 0.032）。发现促卵泡激素（FSH）对培养的猪颗粒细胞产生纤溶酶原激活物没有刺激作用。
