van Schothorst M, Renaud A M
J Appl Bacteriol. 1985 Sep;59(3):223-30. doi: 10.1111/j.1365-2672.1985.tb01783.x.
Large numbers of competitive bacteria may hinder the isolation of salmonellas from food and environmental samples when a pre-enrichment method is used. The addition of 0.1 g/l of malachite green (MG) to buffered peptone water (BPW) inhibited the multiplication of Gram-positive bacteria. Brilliant green had a similar effect but only when the normal recommended concentration of 0.02 g/l was raised to 0.05 g/l. Pure strains of salmonellas were inhibited by MG in BPW, but addition of non fat dried milk (NFDM) (5 g/l or more) counteracted this effect. MG did not affect the recovery of salmonellas injured by heat, freezing, low water activity or acidity in BPW with NFDM. It was concluded that addition of MG to BPW may improve the possibility of isolating salmonellas from heavily contaminated materials by limiting the competitive growth of Gram-positive bacteria and the subsequent lowering of the pH of the broth.
当采用预富集方法时，大量具有竞争力的细菌可能会阻碍从食品和环境样本中分离出沙门氏菌。向缓冲蛋白胨水（BPW）中添加0.1 g/l的孔雀石绿（MG）可抑制革兰氏阳性菌的繁殖。亮绿也有类似效果，但只有当正常推荐浓度0.02 g/l提高到0.05 g/l时才有效。BPW中的MG会抑制沙门氏菌纯菌株的生长，但添加脱脂奶粉（NFDM）（5 g/l或更高）可抵消这种影响。MG不会影响BPW中因热、冷冻、低水分活度或酸度而受损的沙门氏菌的回收率。得出的结论是，向BPW中添加MG可以通过限制革兰氏阳性菌的竞争性生长以及随后降低肉汤的pH值，提高从严重污染材料中分离沙门氏菌的可能性。
