Horiuchi Tae, Furukawa Kyohei, Kikusato Motoi
Laboratory of Animal Nutrition, Graduate School of Agricultural Science, Tohoku University, Aramaki Aza-Aoba, Sendai 980-8572, Japan.
Laboratory of Animal Nutrition, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Vet Sci. 2024 Sep 2;11(9):405. doi: 10.3390/vetsci11090405.
This study aimed to evaluate the suppressive effects of β-hydroxybutyrate (BHB) administration on lipopolysaccharide (LPS)-induced inflammation in broiler chickens.
Twenty-day-old male broiler chickens were randomly allocated to three groups, each of which was treated with saline (control), intraperitoneal administration of LPS [1.5 mg/kg body weight (BW), O127:B8], or LPS plus BHB (3 mmol/kg BW).
Plasma albumin and total protein concentration were significantly reduced by LPS administration, while BHB co-treatment partially attenuated the effects. The LPS treatment significantly induced plasma aspartate and alanine aminotransferase activities, and interleukin (IL)-6 concentration, with the increases suppressed by BHB co-treatment ( < 0.05). The LPS treatment significantly increased the gene expression levels of IL-1β, IL-6, and IL-18 in the spleen and peripheral blood monocytes (PBMC), while the increases were partially attenuated by BHB in the spleen. Relatively higher levels of BHB dehydrogenase 1 and succinyl-CoA:3-ketoacid CoA transferase were observed in the spleen and skeletal muscle, while these gene levels were lower in PBMC and the liver.
The present results suggest that BHB can suppress LPS-induced inflammation, in which ketolytic enzyme expression levels may be involved in broiler chickens.
本研究旨在评估给予β-羟基丁酸（BHB）对脂多糖（LPS）诱导的肉鸡炎症的抑制作用。
将20日龄雄性肉鸡随机分为三组，每组分别用生理盐水（对照组）、腹腔注射LPS[1.5毫克/千克体重（BW），O127:B8]或LPS加BHB（3毫摩尔/千克体重）处理。
给予LPS显著降低了血浆白蛋白和总蛋白浓度，而BHB联合处理部分减轻了这些影响。LPS处理显著诱导了血浆天冬氨酸和丙氨酸转氨酶活性以及白细胞介素（IL）-6浓度，BHB联合处理抑制了这些升高（P<0.05）。LPS处理显著增加了脾脏和外周血单核细胞（PBMC）中IL-1β、IL-6和IL-18的基因表达水平，而BHB在脾脏中部分减轻了这些增加。在脾脏和骨骼肌中观察到相对较高水平的BHB脱氢酶1和琥珀酰辅酶A：3-酮酸辅酶A转移酶，而这些基因水平在PBMC和肝脏中较低。
目前的结果表明，BHB可以抑制LPS诱导的炎症，其中解酮酶表达水平可能参与了肉鸡的炎症反应。
