College of Animal Science and Technology, Shihezi University, Xinjiang 832000, People's Republic of China.
Poult Sci. 2019 Apr 1;98(4):1648-1657. doi: 10.3382/ps/pey532.
This study was carried out to investigate the protective effects of leonurine hydrochloride (LH, from Leonurus sibiricus) on lipopolysaccharide (LPS)-stimulated broiler chicks. A total of 120 one-day-old male Ross broilers were randomly divided into 4 treatment groups with 6 replicates of 5 birds per cage. The experiment was designed as a 2 × 2 factorial arrangement with LH (0 or 120 mg/kg) and LPS (injection of saline or 1.5 mg/kg body weight) levels as treatments. On days 14, 16, 18, and 20 of the trial, broilers were intraperitoneally injected with LPS or saline. Blood, spleen, and liver samples were collected on days 21 and 28 for analysis. The results showed that dietary LH had no effect on growth performance or immunoglobulin concentrations in the serum. However, dietary LH prevented LPS-induced reductions in average daily gain and average daily feed intake in the broilers on days 15-21 of the trial (P > 0.05). Dietary LH supplementation dramatically attenuated the LPS-induced increases in the spleen index, reduced glutathione (GSH) activity (serum and liver) and total superoxide dismutase (T-SOD) activity (serum and spleen), and significantly reduced malondialdehyde (MDA) levels (serum, spleen, and liver) on days 21 and 28 (P < 0.05). Additionally, LH supplementation significantly mitigated the LPS-induced increases in the tumor necrosis factor (TNF)-α (serum and spleen), interleukin (IL)-1β (serum, spleen and liver), IL-2 (liver), IL-6 (serum, spleen and liver), toll-like receptor 4 (TLR4) (spleen and liver), and nuclear factor (NF)-κB (spleen and liver) levels on days 21 and 28 (P < 0.05). Therefore, this study revealed that LH could downregulate the expression of proinflammatory factors, mainly by inhibiting the expression of TLR4 and the activation of NF-κB. LH may be a potential feed additive with dual efficacy as an anti-inflammatory and antioxidant agent.
本研究旨在探讨盐酸毛喉素（LH，来自益母草）对脂多糖（LPS）刺激的肉鸡的保护作用。将 120 只 1 日龄雄性罗斯肉鸡随机分为 4 个处理组，每个处理组 6 个重复，每个重复 5 只鸡。试验设计为 LH（0 或 120mg/kg）和 LPS（注射生理盐水或 1.5mg/kg 体重）水平的 2×2 因子安排。在试验的第 14、16、18 和 20 天，肉鸡腹膜内注射 LPS 或生理盐水。在第 21 和 28 天收集血液、脾脏和肝脏样本进行分析。结果表明，日粮 LH 对生长性能或血清免疫球蛋白浓度没有影响。然而，日粮 LH 可防止 LPS 诱导的试验第 15-21 天肉鸡平均日增重和平均日采食量下降（P＞0.05）。日粮 LH 补充剂可显著减弱 LPS 诱导的脾脏指数升高，降低还原型谷胱甘肽（GSH）活性（血清和肝脏）和总超氧化物歧化酶（T-SOD）活性（血清和脾脏），并显著降低丙二醛（MDA）水平（血清、脾脏和肝脏）在第 21 和 28 天（P＜0.05）。此外，LH 补充剂可显著减轻 LPS 诱导的肿瘤坏死因子（TNF）-α（血清和脾脏）、白细胞介素（IL）-1β（血清、脾脏和肝脏）、IL-2（肝脏）、IL-6（血清、脾脏和肝脏）、Toll 样受体 4（TLR4）（脾脏和肝脏）和核因子（NF）-κB（脾脏和肝脏）水平升高在第 21 和 28 天（P＜0.05）。因此，本研究表明 LH 可以下调促炎因子的表达，主要通过抑制 TLR4 的表达和 NF-κB 的激活。LH 可能是一种具有抗炎和抗氧化双重功效的潜在饲料添加剂。
