Tuo Xiaoqian, Chen Jialan, Hao Cuipei, Dai Xiaole, Zhu Jiayi, Tian Siqi, Zhang Yan, Wang Fan
Department of Gynecology, Shaanxi Provincial People's Hospital, 256 West Youyi Road, Xi'an, 710068, Shaanxi, People's Republic of China.
Discov Oncol. 2024 Sep 27;15(1):489. doi: 10.1007/s12672-024-01382-6.
GPNMB is a type I transmembrane protein, and emerging evidence supports the relationship between GPNMB and cancers.
Through a comprehensive pan-cancer analysis, we examined the expression levels, prognostic significance, and mutation profiles of GPNMB in different cancer types. Subsequently, utilizing in vitro experiments, we elucidated the impact of GPNMB in endometrial cancer (EC).
TIMER2, GEPIA2, UALCAN and cBioPortal were used to analyze the expression pattern, prognostic values, and mutation status of GPNMB. HEC-1B and Ishikawa cells were used to conduct in vitro analyses of GPNMB overexpression. GeneMANIA and TIMER2 were used to evaluate the potential functions and correlations between GPNMB expression and tumor-infiltrating immune cells in EC.
GPNMB was found to be highly expressed in multiple cancers, where it was associated with poor prognosis. Additionally, GPNMB was downregulated at both mRNA and protein levels in EC. Overexpression of GPNMB inhibited the proliferation, migration, and invasion of HEC-1B and Ishikawa cells. Functional analysis showed that GPNMB was enriched in pathways associated with regulation of plasma lipoprotein particle levels. The expression of GPNMB was positively connected with B cell, CD8+ T cell, CD4+ T cell, Macrophage, Neutrophil, and Dendritic cell levels.
Through pan-cancer analysis, we identified the antitumor effect of GPNMB in EC and predicted the potential mechanisms between GPNMB expression and EC.
GPNMB是一种I型跨膜蛋白，越来越多的证据支持GPNMB与癌症之间的关系。
通过全面的泛癌分析，我们研究了GPNMB在不同癌症类型中的表达水平、预后意义和突变谱。随后，利用体外实验，我们阐明了GPNMB在子宫内膜癌（EC）中的作用。
使用TIMER2、GEPIA2、UALCAN和cBioPortal分析GPNMB的表达模式、预后价值和突变状态。使用HEC-1B和Ishikawa细胞对GPNMB过表达进行体外分析。使用GeneMANIA和TIMER2评估GPNMB表达与EC中肿瘤浸润免疫细胞之间的潜在功能和相关性。
发现GPNMB在多种癌症中高表达，与预后不良相关。此外，GPNMB在EC的mRNA和蛋白质水平均下调。GPNMB过表达抑制了HEC-1B和Ishikawa细胞的增殖、迁移和侵袭。功能分析表明，GPNMB在与血浆脂蛋白颗粒水平调节相关的途径中富集。GPNMB的表达与B细胞、CD8 + T细胞、CD4 + T细胞、巨噬细胞、中性粒细胞和树突状细胞水平呈正相关。
通过泛癌分析，我们确定了GPNMB在EC中的抗肿瘤作用，并预测了GPNMB表达与EC之间的潜在机制。
