Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, 59 Cangwu Road, Haizhou, Lianyungang, 222005, China.
Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, 59 Cangwu Road, Haizhou, Lianyungang, 222005, China.
Biotechnol Lett. 2024 Dec;46(6):1027-1035. doi: 10.1007/s10529-024-03533-9. Epub 2024 Sep 27.
This investigation probes the role of the electron mediator, neutral red (NR), in the electrosynthesis process, specifically examining its effect on the production of succinic acid by Actinobacillus succinogenes. Our findings reveal that NR, when integrated into the cell membrane, is pivotal for sustaining MEC efficiency. Nevertheless, it is susceptible to both intrinsic and MECs-induced degradation. Notably, during the exponential growth phase of the bacteria, NR is readily incorporated into the cell membrane. However, the supplemental addition of NR fails to significantly enhance the MEC's capacity for succinic acid synthesis, no matter what stage of bacterial growth. And significant depletion of membrane-associated NR is not adequately compensated by the NR present in the fermentation liquid. The ORP feedback-regulated MECs adeptly conserve the NR on the cell membrane, which is essential for maintaining the efficiency of long-term electrosynthesis. The presence of NR on the cell membrane is essential for the functionality of MECs, yet its external replenishment hard. Implementing precise electro-potential regulation strategies can effectively diminish the degradation of NR, thus maintaining the system's efficiency.
本研究探讨了电子媒介中性红（NR）在电合成过程中的作用，特别考察了其对产琥珀酸谷氨酸棒杆菌生产琥珀酸的影响。我们的研究结果表明，NR 整合到细胞膜中对于维持 MEC 效率至关重要。然而，它容易受到内在因素和 MECs 诱导的降解。值得注意的是，在细菌的指数生长阶段，NR 很容易被整合到细胞膜中。然而，NR 的补充添加并不能显著提高 MEC 合成琥珀酸的能力，无论细菌生长的哪个阶段。并且，膜相关 NR 的大量消耗不能被发酵液中存在的 NR 充分补偿。ORP 反馈调节的 MECs 能够巧妙地将 NR 保存在细胞膜上，这对于维持长期电合成的效率是必不可少的。NR 在细胞膜上的存在对于 MECs 的功能至关重要，但外部补充却很困难。实施精确的电势能调节策略可以有效地减少 NR 的降解，从而维持系统的效率。
