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基于冷冻置换和传统透射电子显微镜的腺叶狸藻腺体和叶细胞的细胞结构

Cyto-architecture of Byblis glands and leaf cells based on freeze-substitution and conventional TEM.

作者信息

Płachno Bartosz J, Lancelle Sue, Świątek Piotr, Hepler Peter K, Weidinger Marieluise, Lichtscheidl Irene

机构信息

Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Kraków, 30-387 Kraków, Poland.

Biology Department, University of Massachusetts Amherst, 221 Morrill Science Center III, Amherst, MA 01003-9297, USA.

出版信息

Ann Bot. 2025 Feb 19;135(3):463-482. doi: 10.1093/aob/mcae173.

DOI:10.1093/aob/mcae173
PMID:39331380
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11897603/
Abstract

BACKGROUND AND AIMS

Byblis liniflora (Byblidaceae) is a carnivorous plant that has developed sticky flypaper traps with two types of glandular trichomes producing digestive enzymes and sticky mucilage. This study aimed to analyse the ultrastructure of these glandular leaf trichomes based on rapid freeze-fixation and conventional chemical fixation in the attempt to understand their functional contribution to the carnivorous performance of the plants.

METHODS

The Byblis cells were studied in transmission electron microscopy, scanning electron microscopy and scanning transmission electron microscopy using cryo-techniques for fixation and substitution in addition to conventional chemical fixation.

KEY RESULTS

We show in detail the architecture of both the digestive glands and the mucilage glands with their relevant sets of organelles. Both mitochondria and plastids have a conspicuous plasticity, with branches and constrictions, and they associate to form clusters. The glandular cells appear to be transfer cells with cell wall ingrowths. Digestive glands occur in different states of development. Their cuticle forms discontinuities that are unique among glands of carnivorous plants. They look like cuticular holes - the cuticle separates from the cell wall in only one spot and then ruptures. Cuticular discontinuities thus differ from the cuticular gaps and cuticular pores so far described in carnivorous plants. We therefore propose for them the term 'cuticular holes'.

CONCLUSIONS

Application of cryo-techniques made it possible to show the true structure of the cell wall and the relationship between cell wall ingrowths and organelles, as well as the morphology and structure of organelles and their associations.

摘要

背景与目的

丝叶腺毛草（腺毛草科）是一种食虫植物，它进化出了粘性的捕虫纸陷阱，带有两种能产生消化酶和粘性粘液的腺毛。本研究旨在基于快速冷冻固定和传统化学固定方法分析这些腺毛的超微结构，以试图了解它们对植物食虫性能的功能贡献。

方法

除了传统化学固定方法外，还使用冷冻技术进行固定和置换，通过透射电子显微镜、扫描电子显微镜和扫描透射电子显微镜对丝叶腺毛草的细胞进行研究。

关键结果

我们详细展示了消化腺和粘液腺的结构及其相关的细胞器组。线粒体和质体都具有明显的可塑性，有分支和缢缩，它们相互关联形成簇。腺细胞似乎是具有细胞壁内突生长的传递细胞。消化腺处于不同的发育状态。它们的角质层形成了食肉植物腺体中独特的不连续结构。它们看起来像角质孔——角质层仅在一个点与细胞壁分离，然后破裂。因此，角质不连续结构不同于迄今为止在食肉植物中描述的角质间隙和角质孔。因此，我们为它们提出“角质孔”这一术语。

结论

冷冻技术的应用使得能够展示细胞壁的真实结构、细胞壁内突生长与细胞器之间的关系，以及细胞器的形态和结构及其关联。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2a/11897603/526ba29c80dc/mcae173_fig15.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2a/11897603/f96a414c2beb/mcae173_fig7.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2a/11897603/eb4b8fef1e35/mcae173_fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2a/11897603/2b1ded45c4c6/mcae173_fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2a/11897603/37ff55082e27/mcae173_fig11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2a/11897603/50b6e326535f/mcae173_fig12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2a/11897603/768372a9c95b/mcae173_fig13.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2a/11897603/526ba29c80dc/mcae173_fig15.jpg

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