Department of Clinical Laboratory, Peoples Hospital of Deyang City, Deyang, China.
Department of Rheumatology, Peoples Hospital of Deyang City, Deyang, China.
Medicine (Baltimore). 2024 Sep 27;103(39):e39827. doi: 10.1097/MD.0000000000039827.
The purpose was to identify transactivation DNA-binding protein-related genes in salivary gland injury in primary Sjögren syndrome (pSS) in southwest China. We downloaded the datasets of GSE7451, GSE23117, and GSE40611. In order to screen the candidate genes, 2 kinds of machine learning algorithms were used. We collected blood from 28 patients and 20 controls to verify the expression of candidate genes using quantitative real-time polymerase chain reaction. The receiver operating characteristic curve was used to evaluate the diagnostic efficiency. Correlations between candidate genes and immune cells were examined. A total of 31 differentially expressed genes were obtained. Through different algorithms, 6 genes including IFIT1, CSF2RB, TRIM22, PPM1H, VAMP7, and C21orf2 were getted. Validation results suggested that the expression of CSF2RB, VAMP7, IFIT1, C21orf2, and TRIM22 was significantly increased in pSS. The area under the curve of CSF2RB was 0.937 and that of TRIM22 was 0.915. Immune infiltration analysis showed that the percentage of activated mast cells was lower than the controls (P = .025). Correlation analysis suggested that CSF2RB was associated with immune cell infiltration. The expression of CSF2RB was significantly upregulated, which could be related to the increase of γδ T cells. We revealed that CSF2RB could be the candidate gene of pSS. CSF2RB was involved by regulating various immune cells. The expression of CSF2RB was significantly upregulated, which was related to the increase of γδ T cells.
目的是鉴定中国西南地区原发性干燥综合征（pSS）唾液腺损伤中反式激活 DNA 结合蛋白相关基因。我们下载了数据集 GSE7451、GSE23117 和 GSE40611。为了筛选候选基因，使用了 2 种机器学习算法。我们收集了 28 名患者和 20 名对照者的血液，使用实时定量聚合酶链反应验证候选基因的表达。使用受试者工作特征曲线评估诊断效率。检查候选基因与免疫细胞之间的相关性。共获得 31 个差异表达基因。通过不同的算法，得到了 6 个基因，包括 IFIT1、CSF2RB、TRIM22、PPM1H、VAMP7 和 C21orf2。验证结果表明，CSF2RB、VAMP7、IFIT1、C21orf2 和 TRIM22 在 pSS 中表达显著增加。CSF2RB 的曲线下面积为 0.937，TRIM22 的曲线下面积为 0.915。免疫浸润分析显示，活化肥大细胞的百分比低于对照组（P=0.025）。相关性分析表明，CSF2RB 与免疫细胞浸润有关。CSF2RB 的表达显著上调，这可能与 γδ T 细胞的增加有关。我们揭示了 CSF2RB 可能是 pSS 的候选基因。CSF2RB 通过调节各种免疫细胞参与其中。CSF2RB 的表达显著上调，这与 γδ T 细胞的增加有关。
