Department of Science in Smart Agriculture Systems, Chungnam National University, Daejeon, Republic of Korea.
Department of Crop Science, Chungnam National University, Daejeon, Republic of Korea.
Sci Data. 2024 Sep 27;11(1):1044. doi: 10.1038/s41597-024-03903-y.
Wasabi (Eutrema japonicum) is a plant belonging to the Brassicaceae family that produces its distinctive pungent taste through allyl isothiocyanate. This study achieved a high-quality chromosome-level genome assembly of the E. japonicum 'Magic' bred in Korea for its rapid growth cycle. The assembly was accomplished using a combination of Illumina, PacBio HIFI, Nanopore MinION, and Pore-C scaffolding technologies. The final assembled genome size is 794.6 Mb, anchored to 14 chromosomes. The genome comprises 67.56% repetitive elements and has a BUSCO score of 99.3%, indicating a high level of completeness. Compared to previously published assemblies with a different cultivar, the total length increased by approximately 48.08 Mb, while the number of Ns decreased from 89,000 to 49,000, and the assembly gaps (500 N padding) reduced from 178 to 98, resulting in a higher quality assembly. This genome will be a valuable resource for genetic and biological research on E. japonicum, aiding in its breeding and genetic improvement.
山葵（Euutrema japonicum）是十字花科植物，通过异硫氰酸烯丙酯产生其特有的刺激性味道。本研究对韩国培育的生长周期快的‘Magic’品种的山葵进行了高质量的染色体水平的基因组组装。该组装使用了 Illumina、PacBio HIFI、Nanopore MinION 和 Pore-C 支架技术的组合。最终组装的基因组大小为 794.6Mb，锚定在 14 条染色体上。该基因组包含 67.56%的重复元件，具有 99.3%的 BUSCO 分数，表明其完整性较高。与以前使用不同品种的组装相比，总长度增加了约 48.08Mb，而 N 的数量从 89000 减少到 49000，组装间隙（500N 填充）从 178 减少到 98，从而得到了更高质量的组装。该基因组将成为研究山葵遗传和生物学的宝贵资源，有助于其繁殖和遗传改良。
