Jang Hoyeol, Cho Ara, Kim Hyuk-Jin, Kim Haneul, Jeong Seung-Hoon, Huh Sun Mi, Yu Hee-Ju, Kim Dong-Kab, Kim Joo-Hwan, Mun Jeong-Hwan
Department of Bioscience and Bioinformatics, Myongji University, Yongin, 17058, Korea.
Division of Forest Biodiversity, Korea National Arboretum, Pocheon, 11186, Korea.
Sci Data. 2024 Dec 18;11(1):1372. doi: 10.1038/s41597-024-04252-6.
Abeliophyllum distichum and Forsythia ovata are closely related species endemic to Korea and are highly valued as ornamental shrubs in the Oleaceae family. A combination of PacBio and Illumina sequencing with Hi-C scaffolding technologies was employed to develop chromosome-level genome assemblies of these species. The assembled genome sizes are 795.72 Mb for A. distichum and 1,108.53 Mb for F. ovata. The assemblies exhibit scaffold N50 lengths of 53.12 Mb and 68.97 Mb, with minimal gaps measuring 323.40 kb and 149.00 kb, and 97.71% and 98.82% BUSCO scores for Embryophyta single-copy orthologs, respectively, indicating high contiguity and completeness. The genomes contain 485.24 Mb and 691.68 Mb of repetitive sequences, 4,926 and 7,175 full-length long terminal repeat retrotransposons, and 49,414 and 57,587 protein-coding genes, respectively. The 14 pseudochromosomes encompass 93.80% of the A. distichum genome and 89.11% of the F. ovata genome, thereby demonstrating one-to-one chromosome-level collinearity. These high-quality genome assemblies serve as invaluable resources for genetic and breeding studies, facilitating a deeper understanding of the evolutionary history of these distinctive species.
朝鲜白鹃梅（Abeliophyllum distichum）和卵叶连翘（Forsythia ovata）是韩国特有的近缘物种，作为木犀科的观赏灌木具有很高的价值。本研究采用PacBio测序、Illumina测序和Hi-C支架技术相结合的方法，构建了这两个物种的染色体水平基因组组装。组装得到的朝鲜白鹃梅基因组大小为795.72 Mb，卵叶连翘基因组大小为1,108.53 Mb。这些组装结果的支架N50长度分别为53.12 Mb和68.97 Mb，最小间隙分别为323.40 kb和149.00 kb，对于陆生植物单拷贝直系同源基因的BUSCO得分分别为97.71%和98.82%，表明具有高连续性和完整性。两个基因组分别包含485.24 Mb和691.68 Mb的重复序列、4,926个和7,175个全长长末端重复反转录转座子，以及49,414个和57,587个蛋白质编码基因。14条假染色体分别覆盖了朝鲜白鹃梅基因组的93.80%和卵叶连翘基因组的89.11%，从而证明了染色体水平的一一对应共线性。这些高质量的基因组组装为遗传和育种研究提供了宝贵的资源，有助于更深入地了解这些独特物种的进化历史。
