数字酶联免疫吸附测定在眼科中的应用。
Applications of Digital Enzyme-Linked Immunosorbent Assays in Ophthalmology.
作者信息
Yu He, Song Jiaping, Li Junrong, Qi Yuanyuan, Fan Zhe, Liu Qiming, Yu Liang, Song Jian, Dong He
机构信息
Department of Ophthalmology, the Third People's Hospital of Dalian, Dalian Medical University, Dalian, China.
Department of Clinical Laboratory, the Third People's Hospital of Dalian, Dalian Medical University, Dalian, China.
出版信息
Cell Biochem Biophys. 2025 Mar;83(1):215-220. doi: 10.1007/s12013-024-01515-2. Epub 2024 Sep 27.
Abstract
Digital enzyme-linked immunosorbent assays (dELISAs) very sensitively detect biomarkers that cannot be measured using traditional methods. The molecules are confined within a small volume, their counts accurately computed, and the results rapidly delivered. Digital ELISAs find many applications. In recent years, such ELISAs have become increasingly used to aid ophthalmological diagnoses and treatments, and have revolutionized the field. This article reviews the applications of dELISAs in clinical practice, especially in the sphere of ophthalmology.
摘要
数字酶联免疫吸附测定（dELISA）能够极其灵敏地检测出无法用传统方法测量的生物标志物。这些分子被限制在一个小体积内，其数量能被精确计算，并且结果能被快速给出。数字ELISA有许多应用。近年来，这种ELISA越来越多地被用于辅助眼科诊断和治疗，并给该领域带来了变革。本文综述了dELISA在临床实践中的应用，尤其是在眼科领域的应用。
相似文献
1
Applications of Digital Enzyme-Linked Immunosorbent Assays in Ophthalmology.数字酶联免疫吸附测定在眼科中的应用。
Cell Biochem Biophys. 2025 Mar;83(1):215-220. doi: 10.1007/s12013-024-01515-2. Epub 2024 Sep 27.
2
Deep learning in ophthalmology: a review.深度学习在眼科学中的应用：综述。
Can J Ophthalmol. 2018 Aug;53(4):309-313. doi: 10.1016/j.jcjo.2018.04.019. Epub 2018 May 30.
3
Development and Validation of Digital Enzyme-Linked Immunosorbent Assays for Ultrasensitive Detection and Quantification of Clostridium difficile Toxins in Stool.用于超灵敏检测和定量粪便中艰难梭菌毒素的数字酶联免疫吸附测定法的开发与验证
J Clin Microbiol. 2015 Oct;53(10):3204-12. doi: 10.1128/JCM.01334-15. Epub 2015 Jul 22.
4
Digital technology, tele-medicine and artificial intelligence in ophthalmology: A global perspective.数字技术、远程医疗和眼科人工智能：全球视角。
Prog Retin Eye Res. 2021 May;82:100900. doi: 10.1016/j.preteyeres.2020.100900. Epub 2020 Sep 6.
5
Bead-free digital enzyme-linked immunoassay on PDMS microwell array.基于聚二甲基硅氧烷（PDMS）微孔阵列的无珠数字酶联免疫分析
Anal Chim Acta. 2025 Jun 22;1356:344045. doi: 10.1016/j.aca.2025.344045. Epub 2025 Apr 8.
6
Review of enzyme-linked immunosorbent assays (ELISAs) for analyses of neonicotinoid insecticides in agro-environments.酶联免疫吸附测定法（ELISA）在分析农业环境中新型烟碱类杀虫剂的应用综述。
J Agric Food Chem. 2013 Dec 26;61(51):12459-72. doi: 10.1021/jf403801h. Epub 2013 Dec 11.
7
Human tear proteomics and peptidomics in ophthalmology: Toward the translation of proteomic biomarkers into clinical practice.眼科中的人类泪液蛋白质组学和肽组学：迈向蛋白质组学生物标志物转化为临床实践。
J Proteomics. 2017 Jan 6;150:359-367. doi: 10.1016/j.jprot.2016.05.006. Epub 2016 May 13.
8
The relationship between toxocara ELISA absorbance values and toxocara ELISA titres: a comparative study.弓蛔虫酶联免疫吸附测定吸光度值与弓蛔虫酶联免疫吸附测定滴度之间的关系：一项对比研究。
Aust N Z J Ophthalmol. 1986 Aug;14(3):199-203. doi: 10.1111/j.1442-9071.1986.tb00036.x.
9
Deep learning in ophthalmology: The technical and clinical considerations.深度学习在眼科学中的技术和临床考虑。
Prog Retin Eye Res. 2019 Sep;72:100759. doi: 10.1016/j.preteyeres.2019.04.003. Epub 2019 Apr 29.
10
[Challenges and prospects in the application of artificial intelligence for ocular disease screening and diagnosis].[人工智能在眼部疾病筛查与诊断中的应用挑战与前景]
Zhonghua Yan Ke Za Zhi. 2024 Jun 11;60(6):484-489. doi: 10.3760/cma.j.cn112142-20231111-00226.
本文引用的文献
1
Neurofilament Light Chain in Aqueous Humor as a Marker of Neurodegeneration in Glaucoma.房水中神经丝轻链作为青光眼神经退行性变的标志物
Clin Ophthalmol. 2023 Aug 2;17:2209-2217. doi: 10.2147/OPTH.S417664. eCollection 2023.
2
Single-molecule immunoassay technology: Recent advances.单分子免疫分析技术：最新进展。
Talanta. 2023 Dec 1;265:124903. doi: 10.1016/j.talanta.2023.124903. Epub 2023 Jul 3.
3
Alternative Complement Pathway Inhibition by Lampalizumab: Analysis of Data From Chroma and Spectri Phase III Clinical Trials.兰帕利单抗对补体替代途径的抑制作用：来自色度和光谱III期临床试验的数据分析
Ophthalmol Sci. 2023 Feb 13;3(3):100286. doi: 10.1016/j.xops.2023.100286. eCollection 2023 Sep.
4
Serum glial fibrillary acidic protein and neurofilament light chain as biomarkers of retinal neurodysfunction in early diabetic retinopathy: results of the EUROCONDOR study.血清神经丝轻链和胶质纤维酸性蛋白作为早期糖尿病视网膜病变视网膜神经功能障碍的生物标志物：EUROCONDOR 研究结果。
Acta Diabetol. 2023 Jun;60(6):837-844. doi: 10.1007/s00592-023-02076-1. Epub 2023 Mar 23.
5
Association of cognitive function with Neurofilament light chain in the aqueous humor of human eye.人眼房水中认知功能与神经丝轻链的关联。
Front Aging Neurosci. 2022 Nov 3;14:1027705. doi: 10.3389/fnagi.2022.1027705. eCollection 2022.
6
Neurofilament light chain: a new marker for neuronal decay in the anterior chamber fluid of patients with glaucoma.神经丝轻链：青光眼患者前房液中神经元降解的一个新标志物。
Br J Ophthalmol. 2023 Oct;107(10):1432-1437. doi: 10.1136/bjo-2021-320828. Epub 2022 Jun 24.
7
Elevated α-synuclein and NfL levels in tear fluids and decreased retinal microvascular densities in patients with Parkinson's disease.帕金森病患者的泪液中 α-突触核蛋白和神经丝轻链水平升高，视网膜微血管密度降低。
Geroscience. 2022 Jun;44(3):1551-1562. doi: 10.1007/s11357-022-00576-6. Epub 2022 Apr 28.
8
High Level of Inflammatory Cytokines in the Tears: A Bridge of Patients with Concomitant Exotropia and Dry Eye.泪液中高水平的炎症细胞因子：共同性外斜视和干眼症患者的桥梁。
Oxid Med Cell Longev. 2021 Oct 7;2021:5662550. doi: 10.1155/2021/5662550. eCollection 2021.
9
Brain-derived Neurotrophic Factor in the Aqueous Humor of Glaucoma Patients.青光眼患者房水中的脑源性神经营养因子。
J Nippon Med Sch. 2021;88(2):128-132. doi: 10.1272/jnms.JNMS.2021_88-305.
10
Comparison of ELISA- and SIMOA-based quantification of plasma Aβ ratios for early detection of cerebral amyloidosis.基于 ELISA 和 SIMOA 的血浆 Aβ 比值定量比较用于脑淀粉样血管病的早期检测。
Alzheimers Res Ther. 2020 Dec 5;12(1):162. doi: 10.1186/s13195-020-00728-w.
Zotero 插件