青光眼患者房水中的脑源性神经营养因子。

Brain-derived Neurotrophic Factor in the Aqueous Humor of Glaucoma Patients.

机构信息

Department of Ophthalmology, Nippon Medical School.

Department of Pediatrics, Nippon Medical School.

出版信息

J Nippon Med Sch. 2021;88(2):128-132. doi: 10.1272/jnms.JNMS.2021_88-305.

DOI:10.1272/jnms.JNMS.2021_88-305

PMID:33980757

Abstract

BACKGROUND

Brain-derived neurotrophic factor (BDNF) may be involved in the pathogenesis of glaucoma. BDNF concentrations reported in previous studies have varied widely, and the concentration of BDNF in aqueous humor is unknown. In this study, BDNF concentrations in the aqueous humor of glaucoma patients and control patients were measured with ELISA kits.

METHODS

This prospective, observational study examined BDNF levels in aqueous humor in 62 eyes of 43 patients who underwent cataract surgery or trabeculectomy (11 glaucoma patients and 32 non-glaucoma cataract patients as controls). BDNF concentrations were examined by 4 different enzyme-linked immunosorbent assay (ELISA) techniques.

RESULTS

The mean ± SD patient age was 72.0 ± 10.1 (range 35 to 87) years. Two of the techniques detected no BDNF in aqueous humor in any samples (n=3 and n=9, respectively); the average value was less than zero. An ultrasensitive ELISA kit did not yield reliable measurements. Finally, in an even more sensitive ELISA (Simoa-HD1), performed by an outside contractor, 25 (54.3%) eyes were below the detection limit, including 20 (55.6%) control and 5 (50%) glaucoma cases. For eyes with detectable BDNF, the overall BDNF concentration was 0.158 pg/mL (n=21): 0.196 pg/mL (n=16) in controls and 0.034 pg/mL (n=5) in glaucoma cases.

CONCLUSIONS

BDNF level in aqueous humor varies widely.

摘要

背景

脑源性神经营养因子（BDNF）可能参与了青光眼的发病机制。既往研究报道的 BDNF 浓度差异很大，且房水中的 BDNF 浓度尚不清楚。本研究采用 ELISA 试剂盒检测了青光眼患者和对照组患者房水中的 BDNF 浓度。

方法

这是一项前瞻性、观察性研究，检测了 43 例接受白内障手术或小梁切除术患者（11 例青光眼患者和 32 例非青光眼白内障患者作为对照组）的 62 只眼中房水的 BDNF 水平。通过 4 种不同的酶联免疫吸附测定（ELISA）技术检测 BDNF 浓度。

结果

患者年龄的平均值±标准差为 72.0±10.1（范围 35 至 87）岁。其中 2 种技术在任何样本中均未检测到房水中的 BDNF（分别为 n=3 和 n=9；平均值均小于 0）。超敏 ELISA 试剂盒也无法获得可靠的测量值。最后，在由外部承包商进行的更灵敏的 ELISA（Simoa-HD1）中，25（54.3%）只眼低于检测限，包括 20（55.6%）例对照和 5（50%）例青光眼。对于可检测到 BDNF 的眼，总体 BDNF 浓度为 0.158 pg/mL（n=21）：对照组为 0.196 pg/mL（n=16），青光眼组为 0.034 pg/mL（n=5）。