Department of Biotechnology, Central University of South Bihar, Gaya, Bihar, 824236, India.
Sci Rep. 2024 Sep 27;14(1):22148. doi: 10.1038/s41598-024-70899-1.
Stevia rebaudiana (Bertoni), commonly known as stevia, is a sought-after natural sweetener, but its conventional propagation methods are slow and inefficient. This study aims to enhance the in vitro culture for stevia by investigating the impact of different Murashige and Skoog (MS) medium salt strengths and plant growth hormones on growth and rebaudioside A content. Apical bud-containing shoot segments were used as explants and cultured on various semi-solid and liquid MS media formulations, incorporating cytokinins (BAP and Kin), auxins (NAA and IAA), and different MS major salt concentrations (MS full, ½ MS, and ¼ MS). Assessments of shoot growth parameters, root formation, and HPLC analysis for rebaudioside A content were conducted. The optimal conditions for in vitro growth was found to be in the ¼ MS + Kin 3 mg/L + NAA 0.1 mg/L (semi-solid) medium, resulting in significantly improved shoot growth and enhanced 30.04% rebaudioside A content. Genetic fidelity of regenerated plants was confirmed using RAPD and ISSR markers. These findings offer valuable insights for optimizing in vitro propagation of stevia and potentially enhancing rebaudioside A content.
甜菊（Bertoni），俗称甜菊，是一种备受追捧的天然甜味剂，但传统的繁殖方法速度慢且效率低。本研究旨在通过研究不同 Murashige 和 Skoog（MS）培养基盐强度和植物生长激素对生长和莱鲍迪甙 A 含量的影响，来提高甜菊的体外培养。以含顶芽的茎段作为外植体，在不同的半固体和液体 MS 培养基配方上进行培养，加入细胞分裂素（BAP 和 Kin）、生长素（NAA 和 IAA）和不同的 MS 主要盐浓度（MS 全、½ MS 和 ¼ MS）。对芽生长参数、生根和 HPLC 分析莱鲍迪甙 A 含量进行评估。结果发现，在 ¼ MS+Kin3mg/L+NAA0.1mg/L（半固体）培养基中，体外生长的最佳条件为显著提高了芽的生长，并提高了 30.04%的莱鲍迪甙 A 含量。利用 RAPD 和 ISSR 标记物对再生植株的遗传保真度进行了确认。这些发现为优化甜菊的体外繁殖和潜在提高莱鲍迪甙 A 含量提供了有价值的见解。
