Salem Jehan, Hassanein Ahmed, El-Wakil Deiaa A, Loutfy Naglaa
Central Laboratory of Genetic Engineering, Botany and Microbiology Department, Faculty of Science, Sohag University, Sohag 82524, Egypt.
Biology Department, Faculty of Science, Jazan University, Jazan 82817, Saudi Arabia.
Plants (Basel). 2022 Feb 11;11(4):498. doi: 10.3390/plants11040498.
The interaction between cytokinin, auxin and GA controlled in vitro shoot multiplication in paulownia was influenced by a medium water potential (Ψ) modulation, where it was modulated using different textures or strengths of MS medium, media of different types (MS, WPM, SH and B5) or NaCl incorporation. The interaction between 2 mg/L BAP and 0.1 mg/L NAA expressed the highest shoot number on each media type, but it was better with media of lower water potential (MS and WPM), and MS medium was the best. Ψ of full-strength semisolid MS medium expressed the highest shoot multiplication. The opposite was detected when Ψ of MS medium was changed using half- or double-strength MS. Ψ of full-strength MS medium in semisolid form resulted in a valuable interaction between 2 mg/L BAP, 0.1 mg/L NAA and 0.1 mg/L GA, leading to efficient shoot formation, and it was associated with an increase in internode length and decrease in stem diameter, which facilitated obtaining synseeds with a high ability to convert. High genetic variation was recorded under long-term culture (14 subcultures). Polymorphism using the ISSR technique was higher than that of RAPD. A further increase in polymorphism was detected when NaCl was used, where five salt-tolerant lines were selected. Some salt-tolerant-selected lines showed one or more amplification products of a specific molecular weight that did not appear in the control. For example, with OPA-07 and OPG-02 RAPD primers, all the salt-tolerant-selected lines showed the appearance of amplification fragments (610 bp and 300 bp, respectively) that were not detected in control.
细胞分裂素、生长素和赤霉素之间的相互作用控制着泡桐的离体芽增殖，这种相互作用受到培养基水势（Ψ）调节的影响，其中水势调节是通过使用不同质地或强度的MS培养基、不同类型的培养基（MS、WPM、SH和B5）或添加NaCl来实现的。2 mg/L苄氨基嘌呤（BAP）和0.1 mg/L萘乙酸（NAA）之间的相互作用在每种培养基类型上表现出最高的芽数，但在水势较低的培养基（MS和WPM）上效果更好，而MS培养基是最佳选择。全强度半固体MS培养基的Ψ表现出最高的芽增殖。当使用半强度或双倍强度的MS改变MS培养基的Ψ时，结果则相反。半固体形式的全强度MS培养基的Ψ导致2 mg/L BAP、0.1 mg/L NAA和0.1 mg/L赤霉素（GA）之间产生有价值的相互作用，从而实现高效的芽形成，并且这与节间长度增加和茎直径减小相关，这有利于获得具有高转化能力的合子胚。在长期培养（14次继代培养）下记录到较高的遗传变异。使用简单序列重复区间（ISSR）技术检测到的多态性高于随机扩增多态性DNA（RAPD）技术。当使用NaCl时检测到多态性进一步增加，从中筛选出了5个耐盐品系。一些耐盐筛选品系显示出特定分子量的一个或多个扩增产物，而这些产物在对照中未出现。例如，使用OPA - 07和OPG - 02 RAPD引物时，所有耐盐筛选品系均显示出对照中未检测到的扩增片段（分别为610 bp和300 bp）的出现。
