泰国一家三级保健医院采集的空气样本中诺如病毒、严重急性呼吸综合征冠状病毒 2 型和博卡病毒的监测。

Surveillance of norovirus, SARS-CoV-2, and bocavirus in air samples collected from a tertiary care hospital in Thailand.

机构信息

Department of Clinical Pathology, Faculty of Medicine Vajira Hospital, Navamindradhiraj University, Bangkok, Thailand.

Department of Microbiology, Faculty of Public Health, Mahidol University, 420/1 Ratchawithi Road, Bangkok, 10400, Thailand.

出版信息

Sci Rep. 2024 Sep 27;14(1):22240. doi: 10.1038/s41598-024-73369-w.

DOI:10.1038/s41598-024-73369-w

PMID:39333786

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11437068/

Abstract

This study aims to determine the presence of norovirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and bocavirus in air samples from a tertiary care hospital in Bangkok, Thailand. Air samples were collected in water using the BioSampler and concentrated using speedVac centrifugation. Based on RT-qPCR, norovirus RNA and SARS-CoV-2 RNA were detected in 13/60 (21.7%) and 3/60 (5.0%) of samples, respectively. One air sample had a weak positivity for both norovirus and SARS-CoV-2 RNAs. Detection rate of norovirus genogroup (G) II (13.3%) was higher than norovirus GI (6.7%). One air sample (1.7%) tested positive for GI and GII. The norovirus GI RNA concentration was 6.0 × 10 genome copies/m. The norovirus GII RNA concentrations ranged from 3.4 × 10 to 5.0 × 10 genome copies/m. Based on RT-nested PCR, norovirus GII was detected in two (3.3%) samples. All samples tested negative for GI RNA and bocavirus DNA. By phylogenetic analysis, GII.17, which is closely related to the outbreak Kawasaki308/JPN/2015 strain, was found in the RT-nested PCR-positive samples. This study highlights the potential of aerosols for norovirus and SARS-CoV-2 transmission and probably cause gastrointestinal and respiratory illnesses, respectively.

摘要

本研究旨在确定泰国曼谷一家三级保健医院空气中是否存在诺如病毒、严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）和博卡病毒。使用生物采样器在水中采集空气样本，并使用超速离心浓缩。基于 RT-qPCR，分别在 13/60（21.7%）和 3/60（5.0%）的样本中检测到诺如病毒 RNA 和 SARS-CoV-2 RNA。有一个空气样本对诺如病毒和 SARS-CoV-2 RNA 均呈弱阳性。诺如病毒基因型（G）II（13.3%）的检出率高于 GI（6.7%）。一个空气样本（1.7%）同时检测到 GI 和 GII。诺如病毒 GI RNA 浓度为 6.0×10 基因组拷贝/m。诺如病毒 GII RNA 浓度范围为 3.4×10 至 5.0×10 基因组拷贝/m。通过 RT-巢式 PCR，在两个（3.3%）样本中检测到诺如病毒 GII。所有样本均为 GI RNA 和 bocavirus DNA 阴性。通过系统进化分析，在 RT-巢式 PCR 阳性样本中发现了与暴发 Kawasaki308/JPN/2015 株密切相关的 GII.17。本研究强调了气溶胶传播诺如病毒和 SARS-CoV-2 的潜力，可能分别导致胃肠道和呼吸道疾病。