Department of Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
BMC Cancer. 2024 Sep 27;24(1):1184. doi: 10.1186/s12885-024-12938-8.
This study analyzed and discussed the characteristics of peripheral blood lymphocytes (PBLs) in patients with endometrial carcinoma (EC) to explore the PBLs' clinical application value.
This single-center case‒control study analyzed the clinical data of patients with EC and uterine fibroids who underwent surgery at the First Affiliated Hospital of Chongqing Medical University between October 2018 and October 2023 retrospectively. The Center for Clinical Molecular Medical Detection of our hospital performed PBLs detection using flow cytometry, and recorded the detection results in the electronic medical records system. Between-group and subgroup comparisons of PBLs in patients with EC were analyzed using t-test or Mann-Whitney U test. The effect of surgery on PBLs in patients with EC was assessed using a paired t-test or the Wilcoxon signed rank test.
The immune function of patients with EC was significantly lower than that of healthy people (P < 0.05) and those with benign uterine diseases (P < 0.05) and was related to body mass index (BMI), hypertension, diabetes, and blood lipids (P < 0.05). In patients with EC, the PBLs counts decreased significantly after surgery (P < 0.05) and more kinds of lymphocytes were affected in the laparoscopic surgery group than in the open surgery group.
With the decrease of PBLs counts, the immune status of patients with EC is impaired. Metabolic syndrome (Mets), including obesity, hypertension, diabetes, and high blood lipids, also affects the immune function of patients with EC. And for EC patients, the effect of laparoscopic surgery is greater than that of open surgery. PBLs has the potential to be one of indicator during the diagnosis and treatment of EC.
This study was retrospectively registered by the Ethics Committee of the First Affiliated Hospital of Chongqing Medical University (approval number K2023-578).
本研究分析并讨论了子宫内膜癌（EC）患者外周血淋巴细胞（PBL）的特征，旨在探讨 PBL 的临床应用价值。
本单中心病例对照研究回顾性分析了 2018 年 10 月至 2023 年 10 月在重庆医科大学第一附属医院接受手术治疗的 EC 患者和子宫肌瘤患者的临床资料。我院临床分子医学检测中心采用流式细胞术检测 PBL，并将检测结果记录在电子病历系统中。采用 t 检验或 Mann-Whitney U 检验比较 EC 患者 PBL 的组间和亚组间差异。采用配对 t 检验或 Wilcoxon 符号秩检验评估手术对 EC 患者 PBL 的影响。
EC 患者的免疫功能明显低于健康人群（P<0.05）和良性子宫疾病患者（P<0.05），且与体重指数（BMI）、高血压、糖尿病和血脂有关（P<0.05）。在 EC 患者中，手术后 PBL 计数明显下降（P<0.05），腹腔镜手术组受影响的淋巴细胞种类多于开腹手术组。
随着 PBL 计数的减少，EC 患者的免疫状态受损。肥胖、高血压、糖尿病和高血脂等代谢综合征（Mets）也会影响 EC 患者的免疫功能。对于 EC 患者，腹腔镜手术的效果大于开腹手术。PBL 有可能成为 EC 诊断和治疗的指标之一。
本研究由重庆医科大学第一附属医院伦理委员会进行回顾性注册（注册号 K2023-578）。
