Li Shun, Liu Yan, Kang Yu, Liu Wei, Wang Weiping, Wang Zhonghua, Xia Xiaoyan, Chen Xiaoyu, Wang Chen, He Xin
College of Agronomy, Hunan Agricultural University, Changsha 410128, China.
Yuelushan Laboratory, Changsha 410128, China.
Antioxidants (Basel). 2024 Aug 26;13(9):1032. doi: 10.3390/antiox13091032.
Low temperature is a common abiotic stress that causes significant damage to crop production. Polyamines (PAs) are a class of aliphatic amine compounds that serve as regulatory molecules involved in plant growth, development, and response to abiotic and biotic stresses. In this study, we found that the exogenous application of two concentrations of spermidine (Spd) significantly enhanced the freezing tolerance of three differently matured rapeseed ( L.) varieties, as manifested by higher survival rates, lower freezing injury indexes, and reduced HO content. RNA-seq and qRT-PCR analyses showed that Spd enhanced the freezing tolerance of rapeseed by regulating genes related to the PA metabolic pathway and antioxidant mechanism, and generally inhibited the expression of genes related to the JA signaling pathway. This study provides a reference basis for understanding the functionality and molecular mechanisms of polyamines in the response of rapeseed to freezing stress.
低温是一种常见的非生物胁迫，会对作物生产造成重大损害。多胺（PAs）是一类脂肪族胺化合物，作为调节分子参与植物生长、发育以及对非生物和生物胁迫的响应。在本研究中，我们发现外源施加两种浓度的亚精胺（Spd）显著提高了三个不同成熟度的油菜（ Brassica napus L.）品种的抗冻性，表现为更高的存活率、更低的冻害指数和更低的丙二醛（MDA）含量。RNA测序（RNA-seq）和实时定量聚合酶链反应（qRT-PCR）分析表明，Spd通过调控与多胺代谢途径和抗氧化机制相关的基因来提高油菜的抗冻性，并且总体上抑制了与茉莉酸（JA）信号通路相关基因的表达。本研究为理解多胺在油菜对冻害胁迫响应中的功能及分子机制提供了参考依据。
