State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Somatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, China.
Biomolecules. 2024 Sep 6;14(9):1129. doi: 10.3390/biom14091129.
Conventional pulp capping materials have limited anti-inflammatory capacity. It is necessary to develop more effective pulp capping material for the treatment of inflamed pulps. Tannic acid (TA) is a natural, water-soluble polyphenol with antimicrobial and anti-inflammatory properties. This study aimed to investigate the effects of a tannin-containing hydroxypropyl chitin hydrogel (HPCH/TA hydrogel) as an innovative pulp capping material. The physicochemical properties of the composite hydrogels were characterized. The effects of HPCH/TA hydrogel as a pulp capping material were evaluated in vitro and in vivo. The underlying mechanism of the anti-inflammatory effects of HPCH/TA hydrogel was explored. The HPCH/TA hydrogel demonstrated favorable temperature sensitivity, injectability, and antibacterial properties. In vitro, the HPCH/TA hydrogel effectively promoted the proliferation of human dental pulp cells and inhibited interleukin-1β, interleukin-6, and tumor necrosis factor-α expression, possibly by suppressing the nuclear factor kappa-B pathway. In vivo, on the fourth day after capping, the HPCH/TA hydrogel group showed lower inflammatory scores compared to the control and iRoot BP Plus (commercial pulp capping material) group. By the sixth week, complete reparative dentin formation was observed in the HPCH/TA hydrogel group, with no difference in thickness compared to the iRoot BP Plus group. Collectively, the HPCH/TA hydrogel holds promise as a bioactive pulp capping material for promoting the repair of inflamed pulp in vital pulp therapy.
传统的牙髓覆盖材料的抗炎能力有限。因此，有必要开发更有效的牙髓覆盖材料来治疗发炎的牙髓。单宁酸（TA）是一种天然的、水溶性多酚，具有抗菌和抗炎特性。本研究旨在探讨一种含单宁的羟丙基壳聚糖水凝胶（HPCH/TA 水凝胶）作为新型牙髓覆盖材料的效果。对复合水凝胶的理化性质进行了表征。评估了 HPCH/TA 水凝胶作为牙髓覆盖材料的体外和体内效果。探讨了 HPCH/TA 水凝胶抗炎作用的潜在机制。HPCH/TA 水凝胶表现出良好的温度敏感性、可注射性和抗菌性能。体外研究表明，HPCH/TA 水凝胶能有效促进人牙髓细胞的增殖，并抑制白细胞介素-1β、白细胞介素-6 和肿瘤坏死因子-α的表达，可能是通过抑制核因子 kappa-B 通路。体内研究结果显示，在覆盖后的第四天，HPCH/TA 水凝胶组的炎症评分明显低于对照组和 iRoot BP Plus（商业性牙髓覆盖材料）组。到第六周时，HPCH/TA 水凝胶组可见完整的修复性牙本质形成，其厚度与 iRoot BP Plus 组无差异。综上所述，HPCH/TA 水凝胶有望成为一种具有生物活性的牙髓覆盖材料，用于促进活髓治疗中发炎牙髓的修复。
