Zhang Jinwu, Peng Chunzi, Lv Maojie, Yang Shisen, Xie Liji, Feng Jiaxun, Wei Yingyi, Hu Tingjun, He Jiakang, Xie Zhixun, Yu Meiling
Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China.
Antibiotics (Basel). 2024 Aug 23;13(9):793. doi: 10.3390/antibiotics13090793.
() is a critical disease affecting broiler health, with as its primary pathogen. compound extract (PHCE), formulated based on traditional Chinese veterinary principles, contains primarily flavonoids with antibacterial, anti-inflammatory, and antioxidant properties. However, PHCE's efficacy against -induced and its underlying mechanism remain unclear. This study employed network pharmacology and molecular docking to predict PHCE's potential mechanisms in treating NE, followed by determining its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against (). Subsequently, the effects of various PHCE doses on intestinal damage, antioxidant capacity, and inflammatory factors in -infected broilers were assessed. Network pharmacology and molecular docking suggested that PHCE's therapeutic mechanism for involves the NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inflammasome signaling pathway, with flavonoids such as quercetin, kaempferol, and isorhamnetin as key active components. PHCE exhibited an MIC of 3.13 mg/mL and an MBC of 12.5 mg/mL against . High PHCE doses effectively reduced intestinal damage scores in both the jejunum and ileum, accompanied by attenuated intestinal pathological changes. Additionally, the high dose significantly increased superoxide dismutase (SOD) levels while decreasing malondialdehyde (MDA), hydrogen peroxide (HO), tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) in the jejunum and ileum ( < 0.01 or < 0.05). PHCE also modulated the expression of caspase-1, IL-1β, gasdermin D (GSDMD), and NLRP3 mRNA, key components of the NLRP3 inflammasome signaling pathway, in both intestinal segments. These findings collectively indicate that PHCE protects against -induced oxidative stress and inflammatory damage in . By enhancing antioxidant capacity, PHCE likely reduces oxidative stress and inflammatory responses, subsequently modulating NLRP3 inflammasome signaling pathway key factor expression. Overall, this research provides valuable insights into the protective mechanism of the herbal compound PHCE and its potential benefits for avian health.
（）是一种影响肉鸡健康的关键疾病，以 为主要病原体。基于中兽医原理配制的复方提取物（PHCE）主要含有具有抗菌、抗炎和抗氧化特性的黄酮类化合物。然而，PHCE对 - 诱导的 及其潜在机制仍不清楚。本研究采用网络药理学和分子对接来预测PHCE治疗坏死性肠炎（NE）的潜在机制，随后确定其对 （）的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。随后，评估了不同剂量的PHCE对感染 的肉鸡肠道损伤、抗氧化能力和炎症因子的影响。网络药理学和分子对接表明，PHCE治疗 的机制涉及NOD样受体热蛋白结构域相关蛋白3（NLRP3）炎性小体信号通路，槲皮素、山奈酚和异鼠李素等黄酮类化合物为关键活性成分。PHCE对 的MIC为3.13 mg/mL，MBC为12.5 mg/mL。高剂量的PHCE有效降低了空肠和回肠的肠道损伤评分，同时减轻了肠道病理变化。此外，高剂量显著提高了空肠和回肠中超氧化物歧化酶（SOD）水平，同时降低了丙二醛（MDA）、过氧化氢（HO）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）（ < 0.01或 < 0.05）。PHCE还调节了两个肠段中NLRP3炎性小体信号通路的关键成分caspase-1、IL-1β、gasdermin D（GSDMD）和NLRP3 mRNA的表达。这些发现共同表明，PHCE可预防 诱导的 氧化应激和炎症损伤。通过增强抗氧化能力，PHCE可能会降低氧化应激和炎症反应，随后调节NLRP3炎性小体信号通路关键因子的表达。总体而言，本研究为草药化合物PHCE的保护机制及其对禽类健康的潜在益处提供了有价值的见解。
