Kim Seongok, Son Bokyung, Kim Hyeryen, Shin Hakdong, Ryu Sangryeol
Department of Food Science & Biotechnology, College of Life Science, Sejong University, Seoul 05006, Republic of Korea.
Carbohydrate Bioproduct Research Center, College of Life Science, Sejong University, Seoul 05006, Republic of Korea.
Antibiotics (Basel). 2024 Aug 24;13(9):799. doi: 10.3390/antibiotics13090799.
is a major food-borne pathogen causing food poisoning. The use of bacteriophages as alternative biocontrol agents has gained renewed interest due to the rising issue of antibiotic-resistant bacteria. We isolated and characterized three phages targeting : SPN3US, SPN3UB, and SPN10H. Morphological and genomic analyses revealed that they belong to the class . SPN3UB, SPN3US, and SPN10H specifically target bacterial surface molecules as receptors, including O-antigens of lipopolysaccharides, flagella, and BtuB, respectively. The phages exhibited a broad host range against strains, highlighting their potential for use in a phage cocktail. Bacterial challenge assays demonstrated significant lytic activity of the phage cocktail consisting of the three phages against UK1, effectively delaying the emergence of phage-resistant bacteria. The phage cocktail effectively reduced contamination in foods, including milk and pork and chicken meats, during cold storage. These results indicate that a phage cocktail targeting different host receptors could serve as a promising antimicrobial strategy to control .
是一种引起食物中毒的主要食源性病原体。由于抗生素耐药细菌问题的日益突出，使用噬菌体作为替代生物防治剂重新引起了人们的兴趣。我们分离并鉴定了三种靶向的噬菌体：SPN3US、SPN3UB和SPN10H。形态学和基因组分析表明它们属于类。SPN3UB、SPN3US和SPN10H分别特异性靶向细菌表面分子作为受体，包括脂多糖的O抗原、鞭毛和BtuB。这些噬菌体对菌株表现出广泛的宿主范围，突出了它们在噬菌体混合物中使用的潜力。细菌挑战试验证明由这三种噬菌体组成的噬菌体混合物对UK1具有显著的裂解活性，有效地延迟了噬菌体抗性细菌的出现。在冷藏期间，噬菌体混合物有效地减少了食品中的污染，包括牛奶、猪肉和鸡肉。这些结果表明，靶向不同宿主受体的噬菌体混合物可作为一种有前途的抗菌策略来控制。
