Snell K
Biochim Biophys Acta. 1985 Dec 13;843(3):276-81. doi: 10.1016/0304-4165(85)90149-7.
Enzymes involved in the pathway of de novo serine biosynthesis (L-phosphoserine aminotransferase) and in alternative pathways of serine utilization (L-serine hydroxymethyltransferase, L-serine dehydratase and L-serine aminotransferase) were assayed in normal adult and fetal rat tissues and in a range of transplantable rat tumors. Serine dehydratase and serine aminotransferase activities were essentially confined to normal adult liver and kidney, whereas phosphoserine aminotransferase and serine hydroxymethyltransferase activities showed a more ubiquitous tissue distribution. In particular, phosphoserine aminotransferase and serine hydroxymethyltransferase activities were appreciable in neoplastic tissues, in the absence of the other enzymes of serine utilization. The pattern of enzyme distribution suggests that the synthesis of serine de novo is metabolically coupled to its utilization for nucleotide biosynthesis in tumors of differing tissue origins.
在正常成年和胎鼠组织以及一系列可移植大鼠肿瘤中，检测了参与从头合成丝氨酸途径（L-磷酸丝氨酸转氨酶）和丝氨酸利用替代途径（L-丝氨酸羟甲基转移酶、L-丝氨酸脱水酶和L-丝氨酸转氨酶）的酶。丝氨酸脱水酶和丝氨酸转氨酶活性主要局限于正常成年肝脏和肾脏，而磷酸丝氨酸转氨酶和丝氨酸羟甲基转移酶活性显示出更广泛的组织分布。特别是，在缺乏其他丝氨酸利用酶的情况下，磷酸丝氨酸转氨酶和丝氨酸羟甲基转移酶活性在肿瘤组织中较为显著。酶分布模式表明，在不同组织起源的肿瘤中，丝氨酸的从头合成在代谢上与其用于核苷酸生物合成的利用相耦合。
