Łata Barbara, Latocha Piotr, Łaźny Radosław, Gutfeld Anna
Section of Basic Research in Horticulture, Department of Plant Protection, Institute of Horticultural Sciences, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.
Department of Environmental Protection and Dendrology, Institute of Horticultural Sciences, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.
Foods. 2024 Sep 15;13(18):2926. doi: 10.3390/foods13182926.
Fruits with a high content of biologically active compounds are essential in preventing many diseases. Therefore, the interest in searching for and testing new plant sources for bioactive constituents remains strong. Although many publications on individual species exist, their results are difficult to compare directly due to varying methods and conditions of analysis. Only a few studies have investigated many different species in a single analysis. Therefore, we examined and compared 21 different genotypes, using various measurement methods for total phenolic content (TPC) (Folin-Ciocalteu, FBBB), total antioxidant capacity (ABTS, FRAP, DPPH), and the HPLC technique for the total ascorbate concentration in freshly harvested fruits. One-way ANOVA, Principal Component Analysis, and Pearson Correlation were used to analyse and compare the results. The tested samples showed significant differences in TPC, ascorbate content, and antioxidant capacity. The correlation between the content of bioactive compounds and antioxidant capacity depended on the analytical methods, with results obtained using the FRAP test being the most strongly correlated. Due to higher levels of polyphenols, ascorbate, and antioxidant potential, the most promising species for further evaluation appear to be × , , , and ×.
富含生物活性化合物的水果对于预防多种疾病至关重要。因此，寻找和测试新的生物活性成分植物来源的兴趣依然浓厚。尽管有许多关于单个物种的出版物，但由于分析方法和条件不同，其结果难以直接比较。只有少数研究在单一分析中考察了许多不同的物种。因此，我们使用多种测量方法，对21种不同的基因型进行了检测和比较，这些方法包括用于测定总酚含量（TPC）的福林-西奥尔特法（Folin-Ciocalteu）、FBBB法，用于测定总抗氧化能力的ABTS法、FRAP法、DPPH法，以及用于测定新鲜收获水果中总抗坏血酸浓度的HPLC技术。采用单因素方差分析、主成分分析和皮尔逊相关性分析来分析和比较结果。测试样品在总酚含量、抗坏血酸含量和抗氧化能力方面存在显著差异。生物活性化合物含量与抗氧化能力之间的相关性取决于分析方法，其中使用FRAP测试获得的结果相关性最强。由于多酚、抗坏血酸和抗氧化潜力含量较高，最有希望进一步评估的物种似乎是×、、和×。
