Zheng Xiang, Wu Fangtong, Zhao Lu, Zhou He, Zhou Zhijun, Jia Zhenhua, Shi Fuming
Laboratory of Enzyme Preparation, Hebei Research Institute of Microbiology Co., Ltd., Baoding 071051, China.
College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
Biology (Basel). 2024 Aug 23;13(9):650. doi: 10.3390/biology13090650.
An insect's gut microbiome is an essential "organ" in their life cycle, playing a crucial role by aiding food digestion and nutrient absorption. This study employed both culture-independent and culture-dependent methods to explore the protease resources present in the gut of the omnivorous insect . The findings revealed that the gut extract of contained a diverse array of proteases, including cysteine proteases, pepsin, serine proteases, and trypsin, as well as some unidentified proteases. Furthermore, the protease gene , derived from gut bacterium DX-3, has been cloned and recombinantly expressed. The recombinant DX-3- protease exhibited a 61.9-fold increase in fermentation level compared to the DX-3 protease. This protease was characterized as a neutral, heat-resistant metalloprotease with an M48 peptidase domain, and it was observed that the binding of Ca to the recombinant protease resulted in the formation of the largest active pocket. This study provides technical support for further development and utilization of functional protein resources in insect gut.
昆虫的肠道微生物群是其生命周期中一个重要的“器官”，通过帮助食物消化和营养吸收发挥关键作用。本研究采用非培养法和培养法来探索杂食性昆虫肠道中存在的蛋白酶资源。研究结果表明，该昆虫肠道提取物含有多种蛋白酶，包括半胱氨酸蛋白酶、胃蛋白酶、丝氨酸蛋白酶和胰蛋白酶，以及一些未鉴定的蛋白酶。此外，源自肠道细菌DX-3的蛋白酶基因已被克隆并进行了重组表达。与DX-3蛋白酶相比，重组DX-3蛋白酶的发酵水平提高了61.9倍。这种蛋白酶被鉴定为具有M48肽酶结构域的中性耐热金属蛋白酶，并且观察到Ca与重组蛋白酶的结合导致形成最大的活性口袋。本研究为进一步开发利用昆虫肠道中的功能蛋白资源提供了技术支持。
