Zheng Xiang, Zhao Lu, Wu Fangtong, Zhou He, Shi Fuming
Laboratory of Enzyme Preparation, Hebei Research Institute of Microbiology Co., Ltd., Baoding 071051, China.
Institute of Life Science and Green Development, College of Life Science, Hebei University, Baoding 071002, China.
Insects. 2024 Aug 21;15(8):629. doi: 10.3390/insects15080629.
The insect gut harbors a diverse array of functional microorganisms that warrant further exploration and utilization. However, there is currently a paucity of research reports on the discovery of protease-producing microorganisms with industrial application value in the gut. Here, we employed microbial culturing to screen and identify the protease-producing microorganisms in the gut extract of . Based on morphological, physiological, and biochemical characterization, 16S rRNA sequencing, as well as ANI and dDDH values of whole genome, the protease-producing strains isolated from the insect gut were identified as DBM-1 and DX-4, DX-3, and DBM-5. According to whole-genome analysis, strain DBM-5, which exhibited the highest enzyme activity, possesses abundant membrane transport genes and carbohydrate metabolism enzymes. In contrast, strains DX-3 and DX-4 not only have the ability to hydrolyze proteins but also demonstrate the capability to hydrolyze plant materials. Furthermore, strains that are closely related tend to have similar metabolic product gene clusters in their genomes. The screening and identification of protease resources are essential for the subsequent development and utilization of gut functional microorganisms and genetic resources in insects.
昆虫肠道中存在着各种各样具有功能的微生物，值得进一步探索和利用。然而，目前关于在肠道中发现具有工业应用价值的产蛋白酶微生物的研究报告较少。在此，我们采用微生物培养方法，对[昆虫名称]肠道提取物中的产蛋白酶微生物进行筛选和鉴定。基于形态学、生理学和生化特征、16S rRNA测序以及全基因组的ANI和dDDH值，从昆虫肠道分离出的产蛋白酶菌株被鉴定为DBM - 1、DX - 4、DX - 3和DBM - 5。根据全基因组分析，酶活性最高的菌株DBM - 5拥有丰富的膜转运基因和碳水化合物代谢酶。相比之下，菌株DX - 3和DX - 4不仅具有水解蛋白质的能力，还表现出水解植物材料的能力。此外，亲缘关系较近的菌株在其基因组中往往具有相似的代谢产物基因簇。蛋白酶资源的筛选和鉴定对于后续昆虫肠道功能微生物和遗传资源的开发利用至关重要。
