Sittichok Sirawut, Passara Hataichanok, Sinthusiri Jirisuda, Moungthipmalai Tanapoom, Puwanard Cheepchanok, Murata Kouhei, Soonwera Mayura
Office of Administrative Interdisciplinary Program on Agricultural Technology, School of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok 10520, Thailand.
Community Public Health Program, Faculty of Public and Environmental Health, Huachiew Chalermprakiet University, Bang Phli, Samut Prakan Province 10540, Thailand.
Insects. 2024 Sep 18;15(9):714. doi: 10.3390/insects15090714.
Monoterpenes are effective and eco-friendly alternatives to conventional chemical larvicides. We tested single and binary mixtures of monoterpenes-geranial and -cinnamaldehyde-for their larvicidal and pupicidal activities against L. and for non-target toxicity on guppies ( Peters), using 1% (/) temephos as a reference. Geranial and -cinnamaldehyde at 250 ppm showed stronger larvicidal and pupicidal activities with a 100% mortality rate and an LT ranging from 0.3 to 0.6 h. All combinations were strongly synergistic against larvae and pupae compared to single formulations, with an increased mortality value (IMV) of 6% to 93%. The combination of geranial + -cinnamaldehyde (1:1) at 200 ppm showed the highest impact, with an IMV of 93%. The strongest larvicidal and pupicidal activities, a 100% mortality rate, and an LT of 0.2 h were achieved by geranial + -cinnamaldehyde (1:1) 500 ppm. They were thirty times more effective than a 1% temephos solution (LT ranging from 6.7 to 96 h) and caused obviously shriveled cuticles and a swollen respiratory system. All single and binary mixtures were not toxic to the guppies. Thus, the combination of geranial + -cinnamaldehyde has great potential as a safe insecticide for controlling mosquito larvae and pupae.
单萜类化合物是传统化学杀幼虫剂有效且环保的替代品。我们测试了单萜类化合物香叶醛和肉桂醛的单一及二元混合物对致倦库蚊的杀幼虫和杀蛹活性以及对孔雀鱼（彼得斯）的非靶标毒性，使用1%（/）的马拉硫磷作为对照。250 ppm的香叶醛和肉桂醛表现出更强的杀幼虫和杀蛹活性，死亡率达100%，致死中时为0.3至0.6小时。与单一制剂相比，所有组合对幼虫和蛹均具有强烈的协同作用，增加死亡率值（IMV）为6%至93%。200 ppm的香叶醛 + 肉桂醛（1:1）组合影响最大，IMV为93%。500 ppm的香叶醛 + 肉桂醛（1:1）实现了最强的杀幼虫和杀蛹活性、100%的死亡率以及0.2小时的致死中时。它们的效果比1%的马拉硫磷溶液（致死中时为6.7至96小时）强30倍，且导致表皮明显萎缩和呼吸系统肿胀。所有单一及二元混合物对孔雀鱼均无毒。因此，香叶醛 + 肉桂醛组合作为控制蚊虫幼虫和蛹的安全杀虫剂具有巨大潜力。
