State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Flower Institution, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China.
Genes (Basel). 2024 Aug 26;15(9):1124. doi: 10.3390/genes15091124.
Cotton aphids ( Glover) cause harm by feeding on phloem sap and spreading plant viruses to lily. Understanding the mechanisms by which aphids infest lily plants is crucial for effective aphid management and control. In this study, we investigated the activity of antioxidants, integrated nontargeted metabolomes and transcriptomes of lilies infested by cotton aphids to explore the changes in lily leaves. Overall, the results indicated that the catalase (CAT) activity in the leaves of the lily plants was greater than that in the leaves of the control plants. A comprehensive identification of 604 substances was conducted in the leaves. Furthermore, the differentially abundant metabolite analysis revealed the enrichment of phenylalanine metabolism and α-linolenic acid metabolism. Moreover, 3574 differentially expressed genes (DEGs), whose expression tended to increase, were linked to glutathione metabolism and phenylpropanoid biosynthesis. In addition, the integrated analysis revealed that the defensive response of lily leaves to aphids is manifested through antioxidant reactions, phenylpropane and flavonoid biosynthesis, and α-linolenic acid metabolism. Finally, the key metabolites were CAT, glutathione, coumaric acid, and jasmonic acid, along with the key genes chalcone synthase (), phenylalanine ammonia-lyase (), and 12-oxo-phytodienoic acid reductase (). Accordingly, the findings of this research elucidate the molecular and metabolic reactions of in lily plants, offering valuable insights for developing aphid resistance strategies in lily farming.
棉蚜（Glover）通过吸食韧皮部汁液和传播植物病毒来危害百合。了解蚜虫侵害百合的机制对于有效防治蚜虫至关重要。本研究通过调查受棉蚜侵害的百合叶片中抗氧化剂的活性，整合非靶向代谢组学和转录组学，以探讨百合叶片的变化。总体而言，结果表明，受棉蚜侵害的百合叶片中的过氧化氢酶（CAT）活性大于对照植株。在叶片中进行了 604 种物质的综合鉴定。此外，差异丰度代谢物分析显示苯丙氨酸代谢和α-亚麻酸代谢的富集。此外，3574 个差异表达基因（DEGs），其表达倾向于增加，与谷胱甘肽代谢和苯丙烷生物合成有关。此外，综合分析表明，百合叶片对蚜虫的防御反应表现为抗氧化反应、苯丙烷和类黄酮生物合成以及α-亚麻酸代谢。最后，关键代谢物为 CAT、谷胱甘肽、香豆酸和茉莉酸，以及关键基因查尔酮合酶（）、苯丙氨酸解氨酶（）和 12-氧-植物二烯酸还原酶（）。因此，本研究阐明了百合植物中 的分子和代谢反应，为百合种植中蚜虫抗性策略的发展提供了有价值的见解。
