Laboratory of Technology and Pharmaceutical Biotechnology (Tecbiofar), Faculty of Pharmacy, Federal University of Rio Grande do Norte, General Gustavo Cordeiro de Farias Avenue, S/N, Petrópolis, Natal 59012-570, Brazil.
Graduate Program of Chemistry, Chemistry Institute, Federal University of Rio Grande do Norte, Senador Salgado Filho Avenue, 3000, Lagoa Nova, Natal 59012-570, Brazil.
Int J Mol Sci. 2024 Sep 13;25(18):9893. doi: 10.3390/ijms25189893.
The rapid resistance developed by pathogenic microorganisms against the current antimicrobial pool represents a serious global public health problem, leading to the search for new antibiotic agents. The scorpion , an abundant species in Northeastern Brazil, presents a rich arsenal of bioactive molecules in its venom, with high potential for biotechnological applications. However, venom cytotoxicity constitutes a barrier to the therapeutic application of its different components. The objective of this study was to produce -venom-loaded cross-linked chitosan nanoparticles (Tsv/CN) at concentrations of 0.5% and 1.0% to improve their biological antimicrobial activity. Polymeric nanoparticles were formed with a homogeneous particle size and spherical shape. Experimental formulation parameters were verified in relation to mean size (<180 nm), zeta potential, polydispersity index and encapsulation efficiency (>78%). Tsv/CN 1.0% demonstrated an ability to increase the antimicrobial venom effect against bacteria, exhibiting an MIC value of 44.6 μg/mL. It also inhibited different yeast species of the genus, and Tsv/CN 0.5% and 1.0% led to a greater inhibitory effect of and strains, presenting MIC values between 22.2 and 5.5 µg/mL, respectively. These data demonstrate the biotechnological potential of these nanosystems to obtain a new therapeutic agent with potential antimicrobial activity.
致病微生物对现有抗菌药物的快速耐药性是一个严重的全球公共卫生问题，促使人们寻找新的抗生素。巴西东北部的蝎子拥有丰富的毒液生物活性分子，具有很高的生物技术应用潜力。然而，毒液的细胞毒性是其不同成分治疗应用的障碍。本研究的目的是制备浓度为 0.5%和 1.0%的载 venom 交联壳聚糖纳米粒子（Tsv/CN），以提高其生物抗菌活性。聚合物纳米粒子具有均匀的粒径和球形形状。实验制剂参数与平均粒径（<180nm）、zeta 电位、多分散指数和包封效率（>78%）相关。Tsv/CN 1.0%显示出增加抗菌毒液对细菌的作用的能力，MIC 值为 44.6μg/mL。它还抑制了不同的酵母属种，并且 Tsv/CN 0.5%和 1.0%对 和 菌株的抑制作用更大，MIC 值分别在 22.2 和 5.5μg/mL 之间。这些数据表明这些纳米系统具有获得具有潜在抗菌活性的新型治疗剂的生物技术潜力。
