Liu Wenjun, Mei Zhuoxin, Yu Lei, Gu Tingting, Li Zhiqiang, Zou Qi, Zhang Shuhui, Fang Hongcheng, Wang Yicheng, Zhang Zongying, Chen Xuesen, Wang Nan
National Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China.
College of Agricultural Science and Technology, Shandong Agricultural and Engineering University, Jinan, Shandong 250100, China.
Hortic Res. 2023 Mar 15;10(5):uhad049. doi: 10.1093/hr/uhad049. eCollection 2023 May.
Anthocyanins are valuable compounds in red-fleshed apples. The MdMYB10 transcription factor is an important regulator of the anthocyanin synthesis pathway. However, other transcription factors are key components of the complex network controlling anthocyanin synthesis and should be more thoroughly characterized. In this study, we used a yeast-based screening technology to identify MdNAC1 as a transcription factor that positively regulates anthocyanin synthesis. The overexpression of in apple fruits and calli significantly promoted the accumulation of anthocyanins. In binding experiments, we demonstrated that MdNAC1 combines with the bZIP-type transcription factor to activate the transcription of and . Our analyses also indicated that the expression of is strongly induced by ABA because of the presence of an ABRE -acting element in its promoter. Additionally, the accumulation of anthocyanins in apple calli co-transformed with and increased in the presence of ABA. Therefore, we revealed a novel anthocyanin synthesis mechanism involving the ABA-induced transcription factor MdNAC1 in red-fleshed apples.
花青素是红肉苹果中的重要化合物。MdMYB10转录因子是花青素合成途径的重要调节因子。然而，其他转录因子是控制花青素合成的复杂网络的关键组成部分，应进行更深入的表征。在本研究中，我们使用基于酵母的筛选技术鉴定出MdNAC1是一种正向调节花青素合成的转录因子。在苹果果实和愈伤组织中过表达显著促进了花青素的积累。在结合实验中，我们证明MdNAC1与bZIP型转录因子结合以激活和的转录。我们的分析还表明，由于其启动子中存在ABRE作用元件，的表达受到ABA的强烈诱导。此外，在ABA存在下，与和共转化的苹果愈伤组织中花青素的积累增加。因此，我们揭示了一种涉及红肉苹果中ABA诱导的转录因子MdNAC1的新的花青素合成机制。
