Facile Synthesis of -(4-Bromo-3-methylphenyl)pyrazine-2-carboxamide Derivatives, Their Antibacterial Activities against Clinically Isolated XDR , Alkaline Phosphatase Inhibitor Activities, and Docking Studies.

The emergence of extensively drug-resistant Typhi (XDR-) poses a grave public health threat due to its resistance to fluoroquinolones and third-generation cephalosporins. This resistance significantly complicates treatment options, underscoring the urgent need for new therapeutic strategies. In this study, we synthesized pyrazine carboxamides (-) in good yields through the Suzuki reaction. Afterward, we evaluate their antibacterial activities against XDR- via the agar well diffusion method; has the strongest antibacterial activity with MIC 6.25 (mg/mL). Moreover, in vitro Alkaline Phosphatase inhibitor activity was also determined; is the most potent compound, with an IC of 1.469 ± 0.02 µM. Further, in silico studies were performed to find the type of interactions between synthesized compounds and target proteins.