da Silva Jaiurte Gomes Martins, de Melo Ismaela Maria Ferreira, Alves Érique Ricardo, de Oliveira Glícia Maria, da Silva Anderson Arnaldo, Cavalcanti Isabela Macário Ferro, Araujo Diego Neves, Pinto Flávia Cristina Morone, de Andrade Aguiar José Lamartine, Wanderley Teixeira Valéria, Coelho Teixeira Álvaro Aguiar
Graduate Program of Animal Bioscience, Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife 52171-900, PE, Brazil.
Department of Medicine, Federal University of Alagoas, Arapiraca 57309-005, AL, Brazil.
Polymers (Basel). 2024 Sep 15;16(18):2611. doi: 10.3390/polym16182611.
The poor healing of diabetic wounds is characterized by prolonged inflammation and decreased collagen deposition. Diabetic patients exhibit changes in the plasma concentrations of pro-inflammatory cytokines, and the role of specific dressings may have an impact on healing. This study aims to evaluate the effects of a combined treatment comprising a bacterial cellulose dressing and melatonin application on the regulation and expression of inflammatory cytokines, VEGF, PCNA, and collagen in the healing of cutaneous wounds of diabetic rats. Pro-inflammatory cytokines, including IL-6, TNF-α, and VEGF, along with PCNA and type I and III collagen, were evaluated after 14 days. Immunohistochemistry showed decreased levels of IL-6, TNF-α, and VEGF, along with an increased expression of PCNA and type I collagen, in the groups treated exclusively with melatonin and bacterial cellulose associated with melatonin compared to the control and the commercial healing agent. It was concluded that treating the skin lesions of diabetic animals supplemented with melatonin using a bacterial cellulose-based dressing has positive effects in regulating the expression of inflammatory cytokines, vascular endothelial growth factor, and collagen, showing that this association could be a viable therapy approach in wound healing.
糖尿病伤口愈合不良的特征是炎症持续时间延长和胶原蛋白沉积减少。糖尿病患者的促炎细胞因子血浆浓度会发生变化，特定敷料的作用可能会对愈合产生影响。本研究旨在评估细菌纤维素敷料联合褪黑素应用对糖尿病大鼠皮肤伤口愈合过程中炎性细胞因子、血管内皮生长因子（VEGF）、增殖细胞核抗原（PCNA）和胶原蛋白的调节及表达的影响。14天后评估促炎细胞因子，包括白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和VEGF，以及PCNA和I型和III型胶原蛋白。免疫组织化学显示，与对照组和市售愈合剂相比，单独使用褪黑素以及与褪黑素联合使用细菌纤维素治疗的组中，IL-6、TNF-α和VEGF水平降低，同时PCNA和I型胶原蛋白表达增加。得出的结论是，使用基于细菌纤维素的敷料治疗补充褪黑素的糖尿病动物皮肤损伤，在调节炎性细胞因子、血管内皮生长因子和胶原蛋白的表达方面具有积极作用，表明这种联合应用可能是伤口愈合的一种可行治疗方法。
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