Pathology, Institute of Tropical Pathology and Public Health, Universidade Federal de Goiás, Goiânia, GO, 74605-050, Brazil.
Laboratory of Nano & Biotechnology, Institute of Tropical Pathology and Public Health, Universidade Federal de Goiás, Goiânia, GO, 74605-050, Brazil.
J Tissue Viability. 2022 Nov;31(4):606-613. doi: 10.1016/j.jtv.2022.07.015. Epub 2022 Aug 23.
This study aimed to evaluate the cicatricial potential of melatonin when applied to wounds of diabetic rats.
The formulation containing melatonin was developed and applied topically to cutaneous wounds of diabetic rats. 48 Wistar rats were used, divided into two groups of 24 diabetic animals each: (i) control group (CG), the animals received topical application of the no-melatonin formulation; (ii) treatment group (TG), the animals received topical application of the melatonin-containing formulation. All animals in each group were treated at four time points: 3, 7, 14, and 21 days. Each subgroup consisted of six animals.
The treatment with melatonin improved wound healing by promoting wound closure earlier than the control group evaluated. Also improved a better resolution of the inflammatory phase observed mainly at 7 days, higher tissue maturation and expressive collagen deposition.
The observed data reveal that the use of melatonin topically could be a promising strategy for the healing of wounds in diabetes. The results of this study elucidate the effects of previously described pathways in which it is proposed that melatonin acts promoting wound healing in diabetes.
本研究旨在评估褪黑素应用于糖尿病大鼠伤口时的瘢痕形成潜力。
开发了含有褪黑素的制剂，并将其局部应用于糖尿病大鼠的皮肤伤口。使用了 48 只 Wistar 大鼠，分为两组，每组 24 只糖尿病动物：（i）对照组（CG），动物接受无褪黑素制剂的局部应用；（ii）治疗组（TG），动物接受含有褪黑素的制剂的局部应用。每组的所有动物在四个时间点接受治疗：3、7、14 和 21 天。每个亚组由六只动物组成。
与对照组相比，褪黑素治疗更早地促进了伤口闭合，从而改善了伤口愈合。在第 7 天，观察到的炎症期也得到了更好的解决，组织成熟度更高，胶原蛋白沉积更明显。
观察到的数据表明，局部使用褪黑素可能是治疗糖尿病伤口的一种有前途的策略。本研究的结果阐明了先前描述的途径的影响，其中提出褪黑素通过促进糖尿病中的伤口愈合来发挥作用。
