Guan Liangjun, Luo Huibin, Liu Siqiong, Ming Xinrong, Hu Mengdie, Luo Lan, Tan Jingyi, Xiao Shunli
School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, China.
Molecules. 2025 Aug 30;30(17):3554. doi: 10.3390/molecules30173554.
L. (JP) has been traditionally used to treat colds with fever, swollen and sore throat, jaundice, malaria and eczema. Studies indicate that lignans constitute the primary bioactive components, yet systematic phytochemical investigations remain limited. Therefore, it is necessary to establish a rapid and effective method to identify the chemical components in JP. In this study, ultra-high-performance liquid chromatography-quadrupole-Exactive Orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap MS) coupled with parallel reaction monitoring (PRM) was used for the first time to investigate JP. Based on chromatographic retention times, MS and MS² data, and bibliography data, a total of 132 compounds were tentatively identified, including 54 lignans, 19 flavonoids, 31 organic acids, 18 alkaloids, and 10 other types of constituents. Among these, 77 compounds are reported for the first time in JP, including 14 potential novel compounds. These results provide valuable reference and data support for the study of pharmacodynamic substances and quality control of this medicinal plant.
传统上，爵床（L. (JP)）被用于治疗感冒发热、咽喉肿痛、黄疸、疟疾和湿疹。研究表明，木脂素是主要的生物活性成分，但系统的植物化学研究仍然有限。因此，有必要建立一种快速有效的方法来鉴定爵床中的化学成分。在本研究中，首次使用超高效液相色谱-四极杆-Exactive轨道阱质谱（UHPLC-Q-Exactive Orbitrap MS）结合平行反应监测（PRM）对爵床进行研究。基于色谱保留时间、MS和MS²数据以及文献数据，初步鉴定出132种化合物，包括54种木脂素、19种黄酮类化合物、31种有机酸、18种生物碱和10种其他类型的成分。其中，77种化合物是首次在爵床中报道，包括14种潜在的新化合物。这些结果为该药用植物的药效物质研究和质量控制提供了有价值的参考和数据支持。
