Department of Human Physiology, School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa.
Molecules. 2024 Sep 17;29(18):4410. doi: 10.3390/molecules29184410.
Natural flavonoids exert many potential health benefits, including anti-hyperglycaemic effects. However, the effects of gossypetin (GTIN) on glucose homeostasis in pre-diabetes have not yet been investigated. This study examined the effects of GTIN on key markers of glucose homeostasis in a diet-induced pre-diabetic rat model. Pre-diabetes was induced by allowing the animals to feed on a high-fat high-carbohydrate (HFHC) diet supplemented with 15% fructose water for 20 weeks. Following pre-diabetes induction, the pre-diabetic animals were sub-divided into five groups ( = 6), where they were either orally treated with GTIN (15 mg/kg) or metformin (MET) (500 mg/kg), both with and without dietary intervention, over a 12-week period. The results demonstrated that animals in the untreated pre-diabetic (PD) control group exhibited significantly higher fasting and postprandial blood glucose levels, as well as elevated plasma insulin concentrations and increased homeostatic model assessment for insulin resistance (HOMA2-IR) index, relative to the non-pre-diabetic (NPD) group. Similarly, increased caloric intake, body weight and plasma ghrelin levels were observed in the PD control group. Notably, these parameters were significantly reduced in the PD animals receiving GTIN treatment. Additionally, glycogen levels in the liver and skeletal muscle, which were disturbed in the PD control group, showed significant improvement in both GTIN-treated groups. These findings may suggest that GTIN administration, with or without dietary modifications, may offer therapeutic benefits in ameliorating glucose homeostasis disturbances associated with the PD state.
天然类黄酮具有许多潜在的健康益处，包括抗高血糖作用。然而，柚皮素（GTIN）对糖尿病前期葡萄糖稳态的影响尚未得到研究。本研究在饮食诱导的糖尿病前期大鼠模型中研究了 GTIN 对葡萄糖稳态关键标志物的影响。糖尿病前期通过允许动物喂食高脂肪高碳水化合物（HFHC）饮食，并补充 15%果糖水 20 周来诱导。糖尿病前期诱导后，将糖尿病前期动物分为五组（每组 6 只），其中一些动物口服 GTIN（15mg/kg）或二甲双胍（MET）（500mg/kg）治疗，同时进行或不进行饮食干预，持续 12 周。结果表明，未经治疗的糖尿病前期（PD）对照组动物的空腹和餐后血糖水平显著升高，血浆胰岛素浓度升高，稳态模型评估的胰岛素抵抗指数（HOMA2-IR）升高，与非糖尿病前期（NPD）组相比。同样，PD 对照组动物的热量摄入、体重和血浆胃饥饿素水平也增加。值得注意的是，接受 GTIN 治疗的 PD 动物的这些参数显著降低。此外，在 PD 对照组中受到干扰的肝脏和骨骼肌中的糖原水平在 GTIN 治疗组中均有显著改善。这些发现可能表明，GTIN 给药，无论是否进行饮食改变，都可能在改善与 PD 状态相关的葡萄糖稳态紊乱方面提供治疗益处。
