Liu Shuping, Li Xiaojie, Zhu Jiani, Jin Yihong, Xia Chuizheng, Zheng Bingsong, Silvestri Cristian, Cui Fuqiang
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China.
Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, Via San Camillo De Lellis, s.n.c., 01100 Viterbo, Italy.
Plants (Basel). 2024 Sep 10;13(18):2539. doi: 10.3390/plants13182539.
Advances in cell fusion technology have propelled breeding into the realm of somatic hybridization, enabling the transfer of genetic material independent of sexual reproduction. This has facilitated genome recombination both within and between species. Despite its use in plant breeding for over fifty years, somatic hybridization has been limited by cumbersome procedures, such as protoplast isolation, hybridized-cell selection and cultivation, and regeneration, particularly in woody perennial species that are difficult to regenerate. This review summarizes the development of somatic hybridization, explores the challenges and solutions associated with cell fusion technology in woody perennials, and outlines the process of protoplast regeneration. Recent advancements in genome editing and plant cell regeneration present new opportunities for applying somatic hybridization in breeding. We offer a perspective on integrating these emerging technologies to enhance somatic hybridization in woody perennial plants.
细胞融合技术的进步已将育种推进到体细胞杂交领域，使遗传物质能够独立于有性繁殖进行转移。这促进了物种内部和物种之间的基因组重组。尽管体细胞杂交在植物育种中已应用了五十多年，但它一直受到繁琐程序的限制，例如原生质体分离、杂交细胞选择与培养以及再生，尤其是在难以再生的多年生木本植物中。本文综述了体细胞杂交的发展，探讨了多年生木本植物细胞融合技术面临的挑战与解决方案，并概述了原生质体再生过程。基因组编辑和植物细胞再生方面的最新进展为在育种中应用体细胞杂交提供了新机遇。我们对整合这些新兴技术以加强多年生木本植物的体细胞杂交提出了展望。
