基于 CRISPR 的基因组编辑及其在木本植物中的应用。
CRISPR-Based Genome Editing and Its Applications in Woody Plants.
机构信息
College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.
College of Plant Protection, Northwest Agriculture and Forestry University, Yangling, Xianyang 712100, China.
出版信息
Int J Mol Sci. 2022 Sep 5;23(17):10175. doi: 10.3390/ijms231710175.
CRISPR/Cas-based genome editing technology provides straightforward, proficient, and multifunctional ways for the site-directed modification of organism genomes and genes. The application of CRISPR-based technology in plants has a vast potential value in gene function research, germplasm innovation, and genetic improvement. The complexity of woody plants genome may pose significant challenges in the application and expansion of various new editing techniques, such as Cas9, 12, 13, and 14 effectors, base editing, particularly for timberland species with a long life span, huge genome, and ploidy. Therefore, many novel optimisms have been drawn to molecular breeding research based on woody plants. This review summarizes the recent development of CRISPR/Cas applications for essential traits, including wood properties, flowering, biological stress, abiotic stress, growth, and development in woody plants. We outlined the current problems and future development trends of this technology in germplasm and the improvement of products in woody plants.
基于 CRISPR/Cas 的基因组编辑技术为生物基因组和基因的定点修饰提供了直接、高效和多功能的方法。CRISPR 技术在植物中的应用在基因功能研究、种质创新和遗传改良方面具有巨大的潜在价值。木本植物基因组的复杂性可能对各种新编辑技术（如 Cas9、12、13 和 14 效应物、碱基编辑）的应用和扩展构成重大挑战，特别是对于具有长寿命、巨大基因组和多倍体的林地物种。因此，许多新的乐观主义者被吸引到基于木本植物的分子育种研究中。本综述总结了 CRISPR/Cas 在木本植物的木材特性、开花、生物胁迫、非生物胁迫、生长和发育等重要性状中的应用的最新进展。我们概述了该技术在木本植物种质资源和产品改良方面的当前问题和未来发展趋势。
Zotero 插件