来自……的新皂苷的去重复化
Dereplication of New Saponins from .
作者信息
Guzzo Francesca, Durán Alexandra G, Rostoll Laura L, Macías Francisco A, Simonet Ana M
机构信息
Allelopathy Group, Department of Organic Chemistry, Institute of Biomolecules (INBIO), School of Science, University of Cádiz, 11510 Puerto Real, Cádiz, Spain.
Department of Environmental Biological and Pharmaceutical Sciences and Technologies-DiSTABiF, University of Campania "Luigi Vanvitelli", Via Vivaldi 43, 81100 Caserta, Italy.
出版信息
Plants (Basel). 2024 Sep 13;13(18):2570. doi: 10.3390/plants13182570.
The genus comprises over 400 species that are known for their diverse applications, which include being sources of fiber, food, and beverages. There has recently been increased interest in exploring the metabolic content of this genus, and in this respect, saponins are the main compounds of interest. Saponins for have not been described to date, and the current work addresses the dereplication of a saponin-rich fraction to identify the structures of six compounds. The dereplication methods involve the use of UPLC-MS analysis, NMR spectroscopy and published data for saponins. A green extraction and isolation provided ten pure saponins. Remarkably, nine of these saponins have not been reported previously, namely (25)-cantalasaponin-1 and bractofuranosides A-H. These compounds were tested for cytotoxic activity. Bractofuranosides B () and G () displayed 57% and 53% cell viability on HeLa cells at 100 µM, respectively.
该属包含400多种植物，以其多样的用途而闻名，这些用途包括作为纤维、食物和饮料的来源。最近，人们对探索该属植物的代谢成分越来越感兴趣，在这方面，皂苷是主要的研究对象。迄今为止，尚未对该属植物的皂苷进行过描述，目前的工作是对富含皂苷的部分进行去重复分析，以确定六种化合物的结构。去重复分析方法包括使用超高效液相色谱-质谱联用（UPLC-MS）分析、核磁共振（NMR）光谱以及已发表的皂苷数据。通过绿色提取和分离得到了十种纯皂苷。值得注意的是，其中九种皂苷此前尚未见报道，即(25)-cantalasaponin-1和bractofuranosides A-H。对这些化合物进行了细胞毒性活性测试。Bractofuranosides B（）和G（）在100µM浓度下对HeLa细胞的细胞活力分别为57%和53%。
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