School of Pharmacy, Taipei Medical University, Taipei, Taiwan.
Planta Med. 2011 Jun;77(9):929-33. doi: 10.1055/s-0030-1250672. Epub 2011 Jan 17.
Two new steroidal saponins, 8 and 10, along with 7 known steroidal sapogenins and saponins (1-7) and a furostanol saponin (9) were isolated from Agave sisalana Perrine ex Engelm. The structures of these two new compounds were identified and characterized by 1D and 2D NMR spectroscopy and mass spectrometry. In addition, acid hydrolysis and GC-FID were used to confirm the sugar moieties of 8 and 10. The cytotoxic effects of 1-10 on MCF-7, NCI-H460, and SF-268 cancer cells were evaluated, and among them, compound 10 proved to be the most cytotoxic with IC₅₀ values of 1.2, 3.8, and 1.5 µM, respectively.
从龙舌兰中分离得到两种新甾体皂苷 8 和 10，以及 7 种已知甾体皂苷元和皂苷（1-7）和一种呋甾烷醇皂苷（9）。通过 1D 和 2D NMR 光谱和质谱鉴定了这两种新化合物的结构。此外，酸水解和 GC-FID 用于确证 8 和 10 的糖部分。评估了 1-10 对 MCF-7、NCI-H460 和 SF-268 癌细胞的细胞毒性作用，其中化合物 10 表现出最强的细胞毒性，IC₅₀ 值分别为 1.2、3.8 和 1.5 μM。
