Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China.
NHC Key Laboratory of Public Nutrition and Health, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Nutrients. 2024 Sep 10;16(18):3050. doi: 10.3390/nu16183050.
Nutritional supplementation enriched with protein and antioxidants has been demonstrated to effectively strengthen skeletal muscle function and mitigate the risk of sarcopenia. Dietary protein has also been a common carrier to establish bioactive delivery system. Therefore, in this study, a Pickering emulsion delivery system for rutin was constructed with whey protein, and its structural characteristics, bioaccessibility, and molecular interactions were investigated. In the in vivo study, zebrafish ( = 10 in each group), which have a high genetic homology to humans, were treated with dexamethasone to induce sarcopenia symptoms and were administered with rutin, whey protein and the Pickering emulsion, respectively, for muscle movement ability evaluation, and zebrafish treated with or without dexamethasone was used as the model and the control groups, respectively. Results showed that the Pickering emulsion was homogeneous in particle size with a rutin encapsulation rate of 71.16 ± 0.15% and loading efficiency of 44.48 ± 0.11%. Rutin in the Pickering emulsion exhibited a significantly higher bioaccessibility than the free form. The interaction forces between rutin and the two components of whey proteins (α-LA and β-LG) were mainly van der Waals forces and hydrogen bonds. After treatment for 96 h, the zebrafish in Picking emulsion groups showed a significantly increased high-speed movement time and frequency, an increased level of ATP, prolonged peripheral motor nerve length, and normalized muscular histological structure compared with those of the model group ( < 0.05). The results of this study developed a new strategy for rutin utilization and provide scientific evidence for sarcopenia prevention with a food-derived resource.
富含蛋白质和抗氧化剂的营养补充剂已被证明能有效增强骨骼肌功能，降低肌少症的风险。膳食蛋白质也常被用作生物活性递送系统的载体。因此，本研究以乳清蛋白为原料，构建了芦丁的 Pickering 乳液递送系统，并对其结构特性、生物利用度和分子相互作用进行了研究。在体内研究中，使用具有与人高度遗传同源性的斑马鱼（每组 10 条），用地塞米松处理诱导肌少症症状，并分别给予芦丁、乳清蛋白和 Pickering 乳液，以评估肌肉运动能力，并用未用地塞米松处理的斑马鱼作为模型和对照组。结果表明，Pickering 乳液粒径均匀，芦丁包封率为 71.16%±0.15%，载药量为 44.48%±0.11%。Pickering 乳液中的芦丁具有显著更高的生物利用度。芦丁与乳清蛋白（α-LA 和 β-LG）的两种成分之间的相互作用力主要为范德华力和氢键。经 96 h 处理后，与模型组相比，Pickering 乳液组的斑马鱼高速运动时间和频率显著增加，ATP 水平升高，外周运动神经长度延长，肌肉组织学结构正常化（<0.05）。本研究开发了一种利用芦丁的新策略，为利用食品源性资源预防肌少症提供了科学依据。
