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与II型限制修饰系统相关的调控基因家族。

A family of regulatory genes associated with type II restriction-modification systems.

作者信息

Tao T, Bourne J C, Blumenthal R M

机构信息

Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.

出版信息

J Bacteriol. 1991 Feb;173(4):1367-75. doi: 10.1128/jb.173.4.1367-1375.1991.

DOI:10.1128/jb.173.4.1367-1375.1991
PMID:1995588
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207272/
Abstract

Restriction-modification systems must be regulated to avoid autorestriction and death of the host cell. An open reading frame (ORF) in the PvuII restriction-modification system appears to code for a regulatory protein from a previously unrecognized family. First, interruptions of this ORF result in a nonrestricting phenotype. Second, this ORF can restore restriction competence to such interrupted mutants in trans. Third, the predicted amino acid sequence of this ORF resembles those of known DNA-binding proteins and includes a probable helix-turn-helix motif. A survey of unattributed ORFs in 15 other type II restriction-modification systems revealed three that closely resemble the PvuII ORF. All four members of this putative regulatory gene family have a common position relative to the endonuclease genes, suggesting a common regulatory mechanism.

摘要

限制修饰系统必须受到调控，以避免宿主细胞的自身限制和死亡。PvuII限制修饰系统中的一个开放阅读框（ORF）似乎编码一种来自此前未被识别家族的调控蛋白。首先，该ORF的中断会导致非限制表型。其次，这个ORF可以在反式作用中恢复此类中断突变体的限制能力。第三，该ORF的预测氨基酸序列与已知的DNA结合蛋白相似，并包含一个可能的螺旋-转角-螺旋基序。对其他15种II型限制修饰系统中未归属的ORF进行的一项调查发现，有三个与PvuII ORF非常相似。这个假定的调控基因家族的所有四个成员相对于内切核酸酶基因都有一个共同的位置，这表明存在一种共同的调控机制。

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