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患者来源细胞和组织的处理、冷冻保存及生物样本库建立方案。

Protocol for the processing, cryopreservation, and biobanking of patient-derived cells and tissues.

作者信息

Pandey Abhimanu, Venkataraman Shweta, Man Si Ming, Talaulikar Dipti

机构信息

Division of Immunology and Infectious Disease, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia; Haematology Translational Research Unit, ACT Pathology, Canberra Health Services, Canberra, ACT 2605, Australia.

Division of Immunology and Infectious Disease, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103287. doi: 10.1016/j.xpro.2024.103287. Epub 2024 Sep 27.

DOI:10.1016/j.xpro.2024.103287
PMID:39340776
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11467654/
Abstract

Biobanking of patient-derived specimens offers unique opportunities for retrospective testing that could potentially contribute to diagnosing and evaluating clinical conditions, advancing personalized medicine and translational biomedical discovery. In this protocol, we detail the collection, processing, and cryopreservation of peripheral blood, bone marrow, and lymph nodes from patients with hematological malignancies. This protocol can be used for multiomics to gain cellular and molecular insights into blood cancers and to test the therapeutic potential of compounds for translational biomedical research. For complete details on the use and execution of this protocol, please refer to Lim et al. and Rijal et al..

摘要

患者来源样本的生物样本库为回顾性检测提供了独特的机会，这可能有助于诊断和评估临床病症、推动个性化医疗以及转化性生物医学发现。在本方案中，我们详细介绍了血液系统恶性肿瘤患者外周血、骨髓和淋巴结的采集、处理及冷冻保存。该方案可用于多组学研究，以深入了解血癌的细胞和分子特征，并测试化合物在转化性生物医学研究中的治疗潜力。有关本方案使用和执行的完整详细信息，请参考Lim等人以及Rijal等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/63e47ed09b2b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/63f784717cf0/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/9fd2e49f6026/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/a22e27bd6c6d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/b85b1c4e04c3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/2410ad305029/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/63e47ed09b2b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/63f784717cf0/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/9fd2e49f6026/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/a22e27bd6c6d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/b85b1c4e04c3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/2410ad305029/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f77/11467654/63e47ed09b2b/gr5.jpg

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本文引用的文献

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Pre-Analytical Evaluation of Streck Cell-Free DNA Blood Collection Tubes for Liquid Profiling in Oncology.用于肿瘤液体分析的Streck游离DNA血液采集管的分析前评估
Diagnostics (Basel). 2023 Mar 29;13(7):1288. doi: 10.3390/diagnostics13071288.
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骨髓中高比例无反应性 B 细胞通过 CD21(-/low)/CD38- 表达表型定义，预示弥漫性大 B 细胞淋巴瘤患者的生存不良。
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Single-Cell Transcriptomics of Human Lymph Node Stroma.人类淋巴结基质的单细胞转录组学
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