双尾目昆虫蒙古蜉蝣 Mongoloraphidia duomilia 的染色体水平基因组组装。

Chromosome-level genome assembly of the snakefly Mongoloraphidia duomilia (Raphidioptera: Raphidiidae).

机构信息

Department of Entomology, China Agricultural University, Beijing, 100193, China.

Department of Biological Sciences, University of Memphis, Memphis, TN, 38152, USA.

出版信息

Sci Data. 2024 Jun 4;11(1):579. doi: 10.1038/s41597-024-03439-1.

DOI:10.1038/s41597-024-03439-1

PMID:38834590

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11150267/

Abstract

Raphidioptera (snakeflies) are a holometabolan order with the least species diversity but play a pivotal role in understanding the origin of complete metamorphosis. Here, we provide an annotated, chromosome-level reference genome assembly for an Asian endemic snakefly Mongoloraphidia duomilia (Yang, 1998) of the family Raphidiidae, assembled using PacBio HiFi and Hi-C data from female specimens. The resulting assembly is 653.56 Mb, of which 97.90% is anchored into 13 chromosomes. The scaffold N50 is 53.50 Mb, and BUSCO completeness is 97.80%. Repetitive elements comprise 64.31% of the genome (366.04 Mb). We identified 599 noncoding RNAs and predicted 11,141 protein-coding genes in the genome (97.70% BUSCO completeness). The new snakefly genome will facilitate comparison of genome architecture across Neuropterida and Holometabola and shed light on the ecological and evolutionary transitions between Neuropterida and Coleopterida.

摘要

蛇蛉目（蛇蛉）是一个全变态的昆虫目，物种多样性最少，但在理解完全变态的起源方面起着关键作用。在这里，我们提供了一个亚洲特有蛇蛉 Mongoloraphidia duomilia（Yang，1998）的染色体水平注释参考基因组组装，该蛇蛉属于蛇蛉科，使用来自雌性标本的 PacBio HiFi 和 Hi-C 数据进行组装。得到的组装基因组大小为 653.56Mb，其中 97.90%锚定在 13 条染色体上。支架 N50 为 53.50Mb，BUSCO 完整性为 97.80%。重复元件占基因组的 64.31%（366.04Mb）。我们在基因组中鉴定了 599 个非编码 RNA 和预测了 11141 个蛋白质编码基因（BUSCO 完整性为 97.70%）。新的蛇蛉基因组将促进对 Neuropterida 和全变态昆虫的基因组结构的比较，并揭示 Neuropterida 和 Coleoptera 之间的生态和进化过渡。