Department of Entomology, China Agricultural University, Beijing, 100193, China.
Department of Biological Sciences, University of Memphis, Memphis, TN, 38152, USA.
Sci Data. 2024 Jun 4;11(1):579. doi: 10.1038/s41597-024-03439-1.
Raphidioptera (snakeflies) are a holometabolan order with the least species diversity but play a pivotal role in understanding the origin of complete metamorphosis. Here, we provide an annotated, chromosome-level reference genome assembly for an Asian endemic snakefly Mongoloraphidia duomilia (Yang, 1998) of the family Raphidiidae, assembled using PacBio HiFi and Hi-C data from female specimens. The resulting assembly is 653.56 Mb, of which 97.90% is anchored into 13 chromosomes. The scaffold N50 is 53.50 Mb, and BUSCO completeness is 97.80%. Repetitive elements comprise 64.31% of the genome (366.04 Mb). We identified 599 noncoding RNAs and predicted 11,141 protein-coding genes in the genome (97.70% BUSCO completeness). The new snakefly genome will facilitate comparison of genome architecture across Neuropterida and Holometabola and shed light on the ecological and evolutionary transitions between Neuropterida and Coleopterida.
蛇蛉目（蛇蛉）是一个全变态的昆虫目，物种多样性最少，但在理解完全变态的起源方面起着关键作用。在这里，我们提供了一个亚洲特有蛇蛉 Mongoloraphidia duomilia（Yang，1998）的染色体水平注释参考基因组组装，该蛇蛉属于蛇蛉科，使用来自雌性标本的 PacBio HiFi 和 Hi-C 数据进行组装。得到的组装基因组大小为 653.56Mb，其中 97.90%锚定在 13 条染色体上。支架 N50 为 53.50Mb，BUSCO 完整性为 97.80%。重复元件占基因组的 64.31%（366.04Mb）。我们在基因组中鉴定了 599 个非编码 RNA 和预测了 11141 个蛋白质编码基因（BUSCO 完整性为 97.70%）。新的蛇蛉基因组将促进对 Neuropterida 和全变态昆虫的基因组结构的比较，并揭示 Neuropterida 和 Coleoptera 之间的生态和进化过渡。
