改良牛磺胆酸盐-亚碲酸盐-明胶琼脂用于改进弧菌属菌种的鉴别。
Modified taurocholate-tellurite-gelatin agar for improved differentiation of Vibrio species.
作者信息
O'Brien M, Colwell R
出版信息
J Clin Microbiol. 1985 Dec;22(6):1011-3. doi: 10.1128/jcm.22.6.1011-1013.1985.
Abstract
A total of 78 strains, representing 21 Vibrio species, were examined by using taurocholate-tellurite-gelatin agar (TTGA) medium and modified TTGA medium containing 4-methylumbelliferyl-beta-D-galactoside (150 micrograms/ml). Modified TTGA medium allowed for simple and direct detection of beta-D-galactosidase (beta-gal) activity. This feature, in conjunction with other differential characteristics of TTGA medium, gave improved differentiation of the vibrios tested. The modified TTGA medium allowed for easy differentiation of Vibrio cholerae (beta-gal+) from Vibrio parahaemolyticus (beta-gal-). The 4-methylumbelliferyl-beta-D-galactoside substrate is inexpensive and very stable. Incorporation into the agar did not affect the performance of TTGA as a differential medium. The assay for beta-gal activity with this substrate was specific and sensitive.
摘要
使用牛磺胆酸盐-亚碲酸盐-明胶琼脂（TTGA）培养基和含有4-甲基伞形酮基-β-D-半乳糖苷（150微克/毫升）的改良TTGA培养基对总共78株代表21种弧菌属的菌株进行了检测。改良TTGA培养基可用于简单直接地检测β-D-半乳糖苷酶（β-半乳糖苷酶）活性。这一特性与TTGA培养基的其他鉴别特征相结合，改善了所测试弧菌的鉴别效果。改良TTGA培养基可轻松区分霍乱弧菌（β-半乳糖苷酶阳性）和副溶血性弧菌（β-半乳糖苷酶阴性）。4-甲基伞形酮基-β-D-半乳糖苷底物价格低廉且非常稳定。将其加入琼脂中不会影响TTGA作为鉴别培养基的性能。用该底物检测β-半乳糖苷酶活性具有特异性和敏感性。
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