Bansal Priyanka Vinod, Mohanty Liza, Raval Chinmay, Gowdar Inderjit M, Chopra Anshul, Gupta Bharti, Makkad Ramanpal S
MDS Prosthodontics and Oral Implantology, Private Practise, Wakad, Pune, India.
Government Medical College and Hospital, Sundargarh, Odisha, India.
J Pharm Bioallied Sci. 2024 Jul;16(Suppl 3):S2194-S2196. doi: 10.4103/jpbs.jpbs_136_24. Epub 2024 Jul 18.
Attempts should be made to identify the molecule-based biomarkers, which give indication about the progression of precancer and malignancy of the oral area.
To evaluate the reliability of methylation of genes of saliva like P16, MGMT, and DAP-K as biomarkers for diagnosis of oral squamous cell carcinoma.
This study included 30 cases of histopathologically proven cases of oral squamous cell carcinoma. This study was conducted to detect demethylation of genes P16, MGMT, and DAP-K in saliva in these patients. Approximately, 1 mL of saliva was collected from each individual. Bisulfite sequencing PCR (BSP) was done with bisulfite conversion-specific primers produced by MethPrimer. The BSP compounds will be quantified on a one percent agarose gel. A fraction of the products obtained in BSP were then analyzed with the help of qMSP.
The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of methylation of genes P16, MGMT, and DAP-K as biomarkers of oral carcinoma were statistically significant. In our research, there were some very important useful findings regarding the possibility of the use of deoxyribonucleic acid methylation as a potential biomarker for the progression of premalignancy and malignancy of oral cavity, which need to be further followed up.
Salivary biomarkers like P16, MGMT, and DAP-K can be used in the diagnosis of oral squamous cell carcinoma.
应努力识别基于分子的生物标志物，这些标志物可为口腔区域癌前病变和恶性肿瘤的进展提供指示。
评估唾液中P16、MGMT和DAP-K等基因甲基化作为口腔鳞状细胞癌诊断生物标志物的可靠性。
本研究纳入30例经组织病理学证实的口腔鳞状细胞癌病例。本研究旨在检测这些患者唾液中P16、MGMT和DAP-K基因的去甲基化情况。从每个个体收集约1 mL唾液。使用MethPrimer生产的亚硫酸氢盐转化特异性引物进行亚硫酸氢盐测序PCR（BSP）。BSP产物将在1%琼脂糖凝胶上进行定量。然后借助qMSP分析BSP中获得的一部分产物。
P16、MGMT和DAP-K基因甲基化作为口腔癌生物标志物的敏感性、特异性、阳性预测值（PPV）和阴性预测值（NPV）具有统计学意义。在我们的研究中，关于将脱氧核糖核酸甲基化用作口腔癌前病变和恶性肿瘤进展的潜在生物标志物的可能性有一些非常重要的有用发现，需要进一步随访。
P16、MGMT和DAP-K等唾液生物标志物可用于口腔鳞状细胞癌的诊断。
