Alharbi Abdulmajeed
Department of Ophthalmology, College of Medicine, Qassim University, Saudi Arabia.
J Pharm Bioallied Sci. 2024 Jul;16(Suppl 3):S2688-S2690. doi: 10.4103/jpbs.jpbs_361_24. Epub 2024 Jul 12.
Pterygium is a common ocular surface disorder characterized by the growth of fibrovascular tissue onto the cornea, leading to discomfort and visual impairment. Various surgical techniques, including conjunctival autografting, amniotic membrane transplantation, and bare sclera excision, are employed in the management of pterygium.
Cultured human conjunctival epithelial cells were subjected to simulated pterygium conditions, mimicking the fibrovascular proliferation observed . Subsequently, different surgical techniques, including conjunctival autografting, amniotic membrane transplantation, and bare sclera excision, were simulated . Cell viability, proliferation, migration, and inflammatory cytokine expression were assessed using various assays, including MTT assay, scratch assay, and enzyme-linked immunosorbent assay (ELISA). Untreated cells served as controls for comparison.
Conjunctival autografting demonstrated superior outcomes in terms of cell viability and proliferation compared to amniotic membrane transplantation and bare sclera excision. Autografted cells exhibited a significantly higher percentage of viable cells and enhanced proliferative capacity compared to cells subjected to other surgical techniques ( < 0.05). Additionally, conjunctival autografting promoted faster cell migration into the defect area, resulting in more rapid wound closure compared to other techniques. Furthermore, reduced expression of inflammatory cytokines, such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), was observed in cells treated with conjunctival autografts compared to other groups.
findings suggest that conjunctival autografting may offer superior outcomes in the management of pterygium compared to amniotic membrane transplantation and bare sclera excision.
翼状胬肉是一种常见的眼表疾病，其特征是纤维血管组织生长到角膜上，导致不适和视力损害。翼状胬肉的治疗采用了多种手术技术，包括结膜自体移植、羊膜移植和单纯巩膜切除术。
将培养的人结膜上皮细胞置于模拟翼状胬肉的条件下，模拟观察到的纤维血管增生。随后，模拟包括结膜自体移植、羊膜移植和单纯巩膜切除术在内的不同手术技术。使用包括MTT法、划痕试验和酶联免疫吸附测定（ELISA）在内的各种测定方法评估细胞活力、增殖、迁移和炎性细胞因子表达。未处理的细胞作为对照进行比较。
与羊膜移植和单纯巩膜切除术相比，结膜自体移植在细胞活力和增殖方面表现出更好的结果。与接受其他手术技术的细胞相比，自体移植的细胞显示出明显更高的活细胞百分比和增强的增殖能力（<0.05）。此外，与其他技术相比，结膜自体移植促进细胞更快地迁移到缺损区域，从而导致伤口更快闭合。此外，与其他组相比，在接受结膜自体移植治疗的细胞中观察到炎性细胞因子如白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的表达降低。
研究结果表明，与羊膜移植和单纯巩膜切除术相比，结膜自体移植在翼状胬肉的治疗中可能提供更好的结果。
